gDNA removal kit

• Removes gDNA from RNA prior to reverse transcription in the same tube
• HL-dsDNase is inactivated without reducing quality or quantity of RNA
• Minimizes pipetting steps and suitable for high throughput experiments

gDNA removal kit uses a heat labile dsDNase to remove gDNA from RNA preps prior to reverse transcription.

The kit is based on a recombinant heat labile dsDNase which is irreversibly inactivated at low temperatures. This enables an inactivation step including heating to moderate temperatures only (58°C), which is gentle enough to preserve both quality and quantity of all present RNA. Reverse transcription can be performed in the same tube, minimizing pipetting steps and reducing hands-on time.

Most  techniques used for RNA isolation yield RNA with significant amounts of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate mRNA quantification. This is particularly a problem in the quantification of low-copy transcripts or small samples. The gDNA removal kit efficiently removes gDNA from RNA preps to levels below the detection limit of RT-qPCR.