LYSO-ID^® Red cytotoxicity kit (GFP-CERTIFIED^®)

• Assay includes unique drug-like dye that rapidly partitions into cells and labels acidic organelles
• Only commercial assay available that allows for long-term cell monitoring of cytotoxic effects
• Multi-well, high-throughput with rapid 10-15 minute dye incubation
• No co-incubation with artificial phospholipid analogs required for detection,  eliminating the potential for confounding dye-associated artifacts
• Monitors lysosome accumulation as a response to prolonged drug treatment
• Quantitative results in as little as 3 hours, including  drug treatment
   

The LYSO-ID^® Red Cytotoxicity Kit monitors dysfunction of lysosomal degradation using a drug-like cationic amphiphilic tracer (CAT) dye that rapidly and selectively stains acidic organelles, and is suitable for monitoring accumulation of lysosomes and lysosome-like structures in live cells.

Mechanism of Action
A cationic amphiphilic tracer (CAT) dye that rapidly partitions into cells in a similar manner as drugs that induce phospholipidosis. This tracer was created by placing titratable groups on the probe (dye), to enable labeling to be expanded into lamellar inclusion bodies of cells pretreated with weakly basic cell-permeant compounds, such as the antimalarial drug chloroquine. Besides lysosomes themselves, the probe can be employed for highlighting lysosome-like organelles under conditions wherein cells produce vacuoles that contain most of the degradative enzymes of the lysosome but are not as acidic as the parent organelle.