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NGAL (human) ELISA kit

BPD-KIT-036

86 citations

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Highly sensitive ELISA, measuring as little as 4 pg/ml of NGAL

Fast results from up to 40 samples in duplicate in just 4 hours per 96 well plate
Highly sensitive measurement, detecting as low as 4 pg/ml of NGAL
Ready-to-use pre-coated plate and individual calibrators save time and reduce errors
Fully quantitative results that surpass semi-quantitative Western blot analysis

A colorimetric immunometric ELISA kit for the measurement of NGAL in human samples. Absorbance is read at 450nm.

NGAL (also known as Lipocalin-2 or Neutrophil Gelatinase-associated Lipocalin) is a small, ~25kDa secreted protein expressed in epithelial tissues throughout the body.

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Product Details

Alternative Name	Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of NGAL in human urine, plasma, serum, tissue extracts, and culture supernatants.
Assay Time	<4 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	1: 12 strips of 8 Microwells each coated with anti-NGAL (96 wells) + frame 2: Sample Diluent 3: NGAL Calibrators 1-8; 0, 10, 25, 50, 100, 250, 500, 1000 pg/mL 4: 25X Wash Solution Concentrate 5: Biotinylated NGAL Antibody 6: HRP-Streptavidin Conjugate 7: TMB Substrate 8: Stop Solution
Sensitivity	4pg/ml (range 10-1000pg/ml)
Species Reactivity	Human
Technical Info / Product Notes	For the manual please click here.
UniProt ID	P80188
Wavelength	450 nm

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Association of urinary excretion rates of uric acid with biomarkers of kidney injury in patients with advanced chronic kidney disease: López Iglesias, A., Blanco Pardo, M., et al.; PLoS One 19, e0304105 (2024), Abstract
The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males: T. Tominaga, et al.; Antioxidants (Basel) 10, 866 (2021), Application(s): NGAL quantification on human urine samples, Abstract — Full Text
Prognostic value of urinary calprotectin, NGAL and KIM-1 in chronic kidney disease: F.S. Seibert, et al.; Kidney Blood Press. Res. 43, 1255 (2018), Application(s): ELISA using human urine, Abstract
Acute kidney injury prediction in cardiac surgery patients by a urinary peptide pattern: a case-control validation study: J. Metzger, et al.; Crit. Care 20, 157 (2016), Abstract — Full Text
An AKI biomarker lipocalin 2 in the blood derives from the kidney in renal injury but from neutrophils in normal and infected conditions: J. Kanda, et al.; Clin. Exp. Nephrol. 19, 99 (2015), Abstract
Urinary neutrophil gelatinase-associated lipocalin predicts kidney outcome and death in patients with cirrhosis and bacterial infections: R. Barreto, et al.; J. Hepatol. 61, 35 (2014), Application(s): ELISA using human urine, Abstract
Prognostic value of serum neutrophil gelatinase-associated lipocalin in metastatic and nonmetastatic colorectal cancer: J. Martí, et al.; World J. Surg. 37, 1103 (2013), Abstract
Discovery and validation of cell cycle arrest biomarkers in human acute kidney injury: K. Kashani, et al.; Crit. Care 17, R25 (2013), Abstract
Differential expression of cancer-associated proteins in breastmilk: W. Qin, et al.; Breastfeed Med. 8, 120 (2013), Abstract
Day-to-day variation of urinary NGAL and rational for creatinine correction: J. Helmersson-Karlqvist, et al.; Clin. Biochem. 46, 70 (2013), Abstract

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NGAL (rat) monoclonal antibody (8)

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Alternative Name	Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
Application	ELISA, IHC
Host	Mouse
Isotype	IgG1κ
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NGAL (rat) monoclonal antibody (15)

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Alternative Name	Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
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NGAL (human) ELISA kit

BPD-KIT-036

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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