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Western Blot analysis of BML-PW8765: Lane 1: MW Marker, Lane 2: HeLa lysate, (Prod. No. ADI-LYC-HL100), Lane 3: HeLa S100 fraction, (Prod. No. BML-SW8750).
This antibody has been used for immunofluorescence. The image was captured on a confocal microscope and is a projection of 17 images taken at 0.4µ intervals through the cell nucleus. The cells are human primary fibroblasts fixed in 4% paraformaldehyde and permeabilised in 0.5% Triton X-100. BML-PW8765 was used at a dilution of 1:1000 and a goat anti-mouse secondary antibody labeled with fluorophore 594 (Molecular Probes). (Data courtesy of Dr. Carol Shiels, Imperial College, London.)
PAI-1 binds to proteasome components in A549 cells. (A,D) Immunoprecipitation-immunoblotting analysis of PAI-1 interaction with proteasome 20S α3 and 19S Rpt3/S6b subunits in A549 cells. A549 cells were transfected with PAI-1 siRNA/non-target (NT) siRNA or transduced with control/PAI-1 expressing viruses and then treated with bleomycin. PAI-1 protein was immunoprecipitated with anti-mouse PAI-1 monoclonal antibody, and Westerns were conducted with specific antibody to 20Sα3 or 19SRpt3/S6b. Proteins on the membrane were stained with Ponceau S to show equal sample loading. (B,C,E,F) Semi-quantification of the band intensities, normalized by the corresponding protein staining band (n = 3).
Image collected and cropped by CiteAb under a CC-BY license from the following publication: PAI-1 Regulation of p53 Expression and Senescence in Type II Alveolar Epithelial Cells. Cells (2023)
PAI-1 binds to proteasome components in A549 cells. (A,D) Immunoprecipitation-immunoblotting analysis of PAI-1 interaction with proteasome 20S α3 and 19S Rpt3/S6b subunits in A549 cells. A549 cells were transfected with PAI-1 siRNA/non-target (NT) siRNA or transduced with control/PAI-1 expressing viruses and then treated with bleomycin. PAI-1 protein was immunoprecipitated with anti-mouse PAI-1 monoclonal antibody, and Westerns were conducted with specific antibody to 20Sα3 or 19SRpt3/S6b. Proteins on the membrane were stained with Ponceau S to show equal sample loading. (B,C,E,F) Semi-quantification of the band intensities, normalized by the corresponding protein staining band (n = 3).
Image collected and cropped by CiteAb under a CC-BY license from the following publication: PAI-1 Regulation of p53 Expression and Senescence in Type II Alveolar Epithelial Cells. Cells (2023)
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RUO – Research Use Only |
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