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AMPIVIEW® RNA probes

Visualize the expression and spatial biology of target genes with ease

AMPIVIEW® RNA probes are uniquely designed with the precision of targeted, sequence specific RNA probes, powered by Enzo’s LoopRNA™ ISH technology to deliver superior sensitivity. When AMPIVIEW® RNA probes hybridize to nucleic acid targets, loops form exposing biotin or digoxigenin labels, making these probes not only the most sensitive means of detection, but adaptable with existing immunohistochemistry (IHC) detection solutions and chromogens. Additionally, morphology of the sample is preserved and the signal of the targeted biomarker can be seen under the light microscope.

Workflow Details

Brochures

AMPIVIEW® RNA Probes

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AMPIVIEW® RNA Probes Benefits

Detection of unique nucleic acid targets (DNA/RNA) down to a single cell level
High sensitivity and reliability
Protocols for formalin-fixed, paraffin-embedded (FFPE) tissues and cells
Mild protocols to preserve tissue morphology
End-user can use existing ISH set ups and protocols
Optimized with Enzo’s cost-effective IHC/ISH detection portfolio
Flexible products ready for any workflow (Manual or Automated)

Applications

Sensitive, Adaptable, Reliable, and Cost-effective Solutions

Basic Research and Drug Discovery/Development

Spatial Biology
Physiological transcription analysis
Disease model studies
Biomarkers research
Target identification and validation
Early preclinical drug discovery analysis
Therapeutics cell response
Companion diagnostic research and development

Clinical Research

Pathogen identification
Biomarker detection
Biomarker validation

Enabling Flexible Multiomics Analysis

Simultaneously analyze multiple targets, including nucleic acids, proteins, and organelles

Spatial Biology

Nucleic Acid Detection

AMPIVIEW® RNA Probes can Detect Copies
of DNA with Sense Probes
and DNA and RNA with Antisense Probes

Detection of HPV 16/18 in SiHa cells (1-3 HPV integrated copies) with A. AMPIVIEW™ HPV 16/18 sense RNA probes to detect viral DNA only and B. AMPIVIEW™ HPV 16/18 antisense RNA probes to detect both viral DNA and viral RNA in cells.

Enzo’s AMPIVIEW® RNA Probes produce high signal and low background

Enzo HPV High Risk antisense probes (RNA)

Leading competitor HPV High Risk probes

Background in the stroma shows non-specific binding and staining

Cervical Squamous Cervical Carcinoma – HPV strong expressing

AMPIVIEW® Loop-RNA probes do not require lengthy protocol to aid the formation of signal structures required in branched DNA probe technology that in turn, tend to increase background.
Dr. Nuovo, Ohio State University

Read the full paper: Nuovo GJ et Al. Appl Immunohistochem Mol Morphol. 2022 Feb 1;30(2):83-90.

AMPIVIEW® RNA Probes are Highly Specific

Detection of SARS-CoV-2 in Lung Tissue

Detection of SARS-CoV-2 RNA. A and B, show the in situ hybridization SARS-CoV-2 RNA in fatal COVID-19 pneumonia with leading competitor and Enzo’s probes. The Competitor probes also signal macrophages (circles, A). C Normal lung tissue show no viral expression with Enzo’s AMPIVIEW® SARS-Cov2 RNA Probes
Nuovo GJ et Al. Appl Immunohistochem Mol Morphol. 2022 Feb 1;30(2):83-90.

SARS-CoV-2-negative tissues show no background staining with Enzo’s AMPIVIEW® RNA probes

AMPIVIEW® SARS-CoV-2 RNA probes

AMPIVIEW® SARS-CoV-2 RNA probes

Competitor SARS-CoV-2 RNA probes

I tested some normal brain and placenta as negative controls for SARS-CoV-2 RNA by ISH with the ACD RNAscope™ and Enzo AMPIVIEW® probes. Note the clean results with the Enzo’s probe. And, also, note the background (red blood cells) with the ACD probes
Dr. Nuovo, Ohio State University

Chromogenic in situ Hybridization E-book

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AMPIVIEW® RNA Probes in situ Hybridization Assay Workflow

From sample preparation to visualization

Permeabilize and Denature

Pre-treatment is performed depending on the sample type:

Fixation of cells or tissue
Deparaffinization (FFPE tissues)
Antigen retrieval
Enzymatic permeabilization

Antigen Retrieval Reagent, pH 6 (10X)
Antigen Retrieval Reagent, pH 9 (10X)
Proteinase K
IHC antigen retrieval reagent (citrate, pH 7.0)
IHC antigen retrieval reagent (citrate, pH 8.0)
IHC enzyme antigen retrieval reagent

Hybridize

AMPIVIEW® RNA probes — Ready-to-Use Probes

AMPIVIEW® RNA probes are designed with Enzo’s LoopRNA ISH™ technology that hybridize to a specific RNA, DNA or RNA/DNA target of interest. Every target probe contains biotin or digoxigenin tags that enable the associated target to be visualized in a specific color chromogens under the light microscope.

AMPIVIEW® HPV 6/11 RNA Probes Set
AMPIVIEW® HPV 16/18 RNA Probes Set
AMPIVIEW® HPV 31/33/51 RNA Probes Set
AMPIVIEW® HPV High-Risk RNA Probes Set
AMPIVIEW^® HPV HR-15 (AS) DNP RNA Probes Set
AMPIVIEW® SARS-CoV-2 RNA Probes Set
AMPIVIEW® SARS-CoV-2 RNA Probes Kit

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Custom Probes

Enzo can design probes for practically ANY gene in ANY genome to be used in ANY tissue or cells.
To order or inquire about custom probes, please fill in the from and a product specialist will contact you.

Detect and Develop

AMPIVIEW® RNA Probes can be detected with Enzo’s IHC/ISH Detection Kits and chromogens.

Detection Solutions

Consistent and reproducible results of AMPIVIEW® RNA probes can be done with Enzo’s linkers and IHC detection solutions.

SAVIEW® PLUS AP reagent
SAVIEW® PLUS HRP reagent
POLYVIEW® PLUS AP (anti-mouse) reagent
POLYVIEW® PLUS AP (anti-rabbit) reagent
POLYVIEW® PLUS HRP (anti-mouse) reagent
POLYVIEW® PLUS HRP (anti-rabbit) reagent
MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green)
Mouse anti-biotin linker (Ready-to-use)
Rabbit anti-biotin linker (Ready-to-use)
DIGX® Mouse anti-digoxigenin linker (Ready-to-Use)
DIGX® Rabbit anti-digoxigenin linker (Ready-to-Use)

Complete Detection Kits

Flexible and complete detection kits from antigen retrieval to counterstain for the detection of AMPIVIEW® RNA Probes.

AMPIVIEW® Dig (anti-mouse) Detection HRP-DAB
AMPIVIEW® Dig (anti-rabbit) Detection AP-Red
AMPIVIEW® Dig (anti-rabbit) Detection HRP-DAB
AMPIVIEW® Dig (anti-mouse) Detection AP-Red
AMPIVIEW® DNP (anti-rabbit) Detection AP-Red
AMPIVIEW® DNP (anti-rabbit) Detection HRP-DAB

Visualize

AMPIVIEW® RNA Probes can be visualized and imaged with a conventional light microscope.

HIGHDEF® DAB Chromogen/Substrate Set
HIGHDEF® Green AP Chromogen/Substrate
HIGHDEF® black IHC chromogen (HRP)
HIGHDEF® blue IHC chromogen (AP)
HIGHDEF® blue IHC chromogen (HRP)
HIGHDEF® red IHC chromogen (AP)
HIGHDEF® yellow IHC chromogen (HRP)
HIGHDEF® Hematoxylin
HIGHDEF® IHC mount

The value of in situ detection of SARS-CoV-2 RNA and proteins in understanding the pathophysiology of severe COVID-19

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AMPIVIEW® RNA probes