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APO-BRDU™ kit

ALX-850-042

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TUNEL assay for the detection of apoptosis by flow cytometry

APO-BRDU™ Kit is a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.

The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br‐dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counter staining the total DNA.

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Product Details

Application	Flow Cytometry
Contents	positive control cells, negative control cells, wash buffer, reaction buffer, TdT enzyme, Br-dUTP, rinse buffer, anti-BrdU mAb – FITC, PI/RNase staining buffer.
Quantity	50 assays.
Technical Info / Product Notes	Br-dUTP is more readily incorporated into the genome of apoptotic cells than are larger ligands like fluorescein, biotin, etc. This greater incorporation coupled with the use of a fluorescein labeled anti-BrdU monoclonal antibody (Prod. No. ALX-804-197L) gives rise to a stronger signal in flow cytometry.

Handling & Storage

Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis): Z. Darzynkiewicz, et al.; Cytometry 27, 1 (1997), (Review), Abstract
Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color: X. Li, et al.; Exp. Cell. Res. 222, 28 (1996), Abstract
Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation: X. Li & Z. Darzynkiewicz; Cell Prolif. 28, 571 (1995), Abstract
Features of apoptotic cells measured by flow cytometry: Z. Darzynkiewicz, et al.; Cytometry 13, 795 (1992), Abstract

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APO-BRDU™ kit

ALX-850-042

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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