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- Highly active MEGACD40L® oligomer mimics in vivo membrane-assisted CD40L aggregation and stimulation without the need for enhancers
- High purity with low endotoxin levels
- Experienced manufacturing results in consistent and dependable lot-to-lot performance
- Optimized formulation for improved stability and enhanced activity
MEGACD40L® is a high activity protein in which two trimeric CD40 ligand molecules are artificially linked via the collagen domain of Adiponectin/ACRP30/AdipoQ. This protein very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations (Prod. No. ALX-850-064).
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B cell lymphocyte activation by MEGACD40L® (Prod. No. ALX-522-110). Method: Peripheral Blood Mononuclear Cells (PBMCs) were incubated at 37°C, 5% CO2 for 48 hours in 48 well plates (1 x 106 cells/well). Each well contained 200µl serum free test media with serially diluted MEGACD40L®. After treatment, cells were washed and dual-stained with mouse anti-human CD19–PE and mouse anti-human CD86–APC and analyzed by flow cytometry. The data are presented as the percent of CD19 positive B cells that co-stain as CD86 positive, at each concentration of MEGACD40L®.
PBMCs (peripheral blood mononuclear cells) were incubated at 37°C, 5% CO2 for 48 hours in 48 well plates (1 x 106 cells per well) containing 200µl serum free test media with serially diluted MEGACD40L® (Prod. No. ALX-522-110), CD40L (Prod. No. ALX-522-015), or CD40L + 2µg/ml Enhancer (Prod. No. ALX-804-034). After treatment the cells were washed 3X with PBS + 1% BSA. The cells were dual stained for 2 hours at 4°C with mouse anti-human CD19 (PE conjugate) and mouse anti-human CD86 (APC conjugate). The cells were washed 2X with PBS + 1% BSA, then re-suspended In PBS. Samples were analyzed on a BD Facs Calibur flow cytometer. The data is presented as the percent of CD86 positive cells per CD19 positive B cells at each concentration.
MEGACD40L® from 7 consecutive manufacturing lots was tested using a B-cell activation assay at 1000ng/mL.
MEGACD40L® was subjected to stability testing by storing the protein at the indicated temperature for 2 months. The protein was then tested for activity using a B cell activation assay. Results indicate MEGACD40L® retains activity under even harsh storage conditions.
Graphical representation of MEGACD40L®, Soluble (human) (recombinant) (Prod. No. ALX-522-110).
| Regulatory Status |
RUO – Research Use Only |
|---|
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| Species Reactivity | Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep |
| Alternative Name | CD154, TNFSF 5, gp39 |
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| Purity | ≥90% (SDS-PAGE) |
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