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CSP polyclonal antibody

ADI-VAP-SV003

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Cysteine string protein (CSP) is a 34 kDa rat brain protein localized to the cytoplasmic surface of the synaptic vesicle. CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking.

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CSP polyclonal antibody Immunohistochemistry — Immunohistochemistry analysis of Xenopus embryonic retina section stained with CSP, pAb.

CSP polyclonal antibody Western blot — Western blot analysis of mouse brain tissue extract, probed with CSP, pAb.

ADI-VAP-SV003 CSP polyclonal antibody Western Blot Human 1 — Presynaptic protein levels in the brains of terminal AD-ANCL patients. a Representative western blots (top) and semi-quantitative analysis (graph, bottom) displaying the protein levels of CSPα in the membrane (M-CSPα) (normalized to Flotillin) and cytosolic/soluble (S-CSPα) (normalized to HSC70) fractions from the occipital lobe of three controls and three terminal AD-ANCL patients. The graphs show the expression level of the indicated proteins normalized to flotillin or HSC70 expression. b Representative western blots (top) and semi-quantitative analysis (graph, bottom) show the protein levels of SNAP-25, vesicle-associated membrane protein 2 (VAMP2/ Synaptobrevin), Syntaxin 1 (STX1), and Synaptophysin (SYP) in the occipital lobe from three controls and three terminal AD-ANCL patients in the membrane fraction. Values represent the mean ± S.E.M. of three independent experiments. **, p ≤ 0.01; ***, p ≤ 0.001 using Student’s t test

Image collected and cropped by CiteAb under a CC-BY license from the following publication: Clinically early-stage CSPα mutation carrier exhibits remarkable terminal stage neuronal pathology with minimal evidence of synaptic loss. Acta Neuropathol Commun (2015)

Product Details

Alternative Name	Cysteine String Protein, DnaJC5
Application	IHC, IP, WB
Application Notes	Detects a band of ~34kDa by Western blot.
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
GenBank ID	U39320
Host	Rabbit
Immunogen	Synthetic peptide corresponding to the sequence near the C-terminus of rat CSP. The sequence is completely conserved in bovine.
Purity Detail	Immunoaffinity purified.
Recommendation Dilutions/Conditions	Immunohistochemistry, Immunoprecipitation (0.5µg/ml)Western Blot (1:2,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Mouse, Rat, Xenopus
UniProt ID	P60905
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The mitochondrial protein Sideroflexin 3 (SFXN3) influences neurodegeneration pathways in vivo.: Ledahawsky, L. M., Terzenidou, M. E., et al.; FEBS J. 289, 3894 (2022), Application(s): WB / Reactant(s): Mouse, Abstract
Adult-Onset Neuronal Ceroid Lipofuscinosis With a Novel DNAJC5 Mutation Exhibits Aberrant Protein Palmitoylation.: Dammer, E. B., Wang, G., et al.; Front. Aging Neurosci. 14, 829573 (2022), Application(s): WB, Abstract
Primary fibroblasts from CSPα mutation carriers recapitulate hallmarks of the adult onset neuronal ceroid lipofuscinosis.: Sands, M. S., Benitez, B. A., et al.; Sci. Rep. 7, 6332 (2017), Application(s): WB, Abstract
Glucosylsphingosine Promotes α-Synuclein Pathology in Mutant GBA-Associated Parkinson’s Disease.: Liu, J., Zhang, X., et al.; J. Neurosci. 37, 9617 (2017), Application(s): IF / Reactant(s): Human, Abstract
Neuronal ceroid lipofuscinosis with DNAJC5/CSPα mutation has PPT1 pathology and exhibit aberrant protein palmitoylation.: Dai, F., Lam, T. T., et al.; Acta Neuropathol. 131, 621 (2016), Application(s): ICC, IHC / Reactant(s): Human, Mouse, Abstract
Clinically early-stage CSPα mutation carrier exhibits remarkable terminal stage neuronal pathology with minimal evidence of synaptic loss: B.A. Benitez, et al.; Acta Neuropathol. Commun. 3, 73 (2015), Application(s): Immunoblotting with human cell lysates, Abstract — Full Text
Oligomerization of Cysteine String Protein alpha mutants causing adult neuronal ceroid lipofuscinosis: Zhang, Y. Q., Chandra, S. S., et al.; Biochim. Biophys. Acta 1842, 2136 (2014), Abstract
CRHSP-28 regulates Ca(2+)-stimulated secretion in permeabilized acinar cells: G.E. Groblewski, et al.; J. Biol. Chem. 276, 28866 (2001), Application(s): WB using rat samples, Abstract
Cysteine string protein, a DnaJ family member, is present on diverse secretory vesicles: R.H. Scheller, et al.; Neuropharmacology 34, 1361 (1995), Application(s): WB using rat samples, Abstract