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Rapid DNA damage ELISA kit for cancer, apoptosis and oxidative stress.
- Highly sensitive measurements, detecting as low as 1 ng/ml
- Reliable and validated in-house in a variety of sample matrices
- Fully quantitative results that surpass semi-quantitative Western blot analysis
- No edge effect allowing you to assay more samples per kit
The DNA Damage EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 2.5 hours. This kit has been validated in-house in a variety of sample matrices and is supplied in a convenient 96 well plate format. Protocol (xls) available upon request.
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage.
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Standard curve of DNA damage ELISA kit
Product Details
| Application |
Colorimetric detection, ELISA |
|---|---|
| Application Notes |
For the measurement of DNA Damage (8-OHdG) in serum, saliva, and urine from any species. Cited sample types include plasma, seminal fluids, cell lysates, culture supernatants and DNA extracts. |
| Assay Time |
<2.5 hours |
| Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
| Contents |
Microtiter plate, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2 |
| Crossreactivity |
The DNA Damage ELISA also detects 8-hydroxyguanosine (product of oxidative RNA damage) and 8-hydroxyguanine (product of oxidative DNA damage by hydroxyl radicals). |
| Sensitivity |
0.59 ng/ml (range 0.94 – 60 ng/ml) |
| Species Reactivity |
Species independent |
| Wavelength |
450 nm |
Handling & Storage
| Use/Stability |
Store all components at 4°C, except standard at -20°C. |
|---|---|
| Shipping |
Dry Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Interactive effects of high n-3 PUFA intake and cyclic heat stress under two dietary antioxidant levels in broiler chickens: Rezar, V., Pal, M. P., et al.; Front. Physiol. 16, 1594095 (2025), Sample Type: Bird (Chicken [G. gallus domesticus]) Serum, Abstract
- Persistent Post COVID-19 Endothelial Dysfunction and Oxidative Stress in Women: Semenova, N. V., Vyrupaeva, E., et al.; Pathophysiology 31, 436 (2024), Sample Type: Human Plasma, Abstract
- Oxidative stress and genotoxicity in oral epithelial cells from subjects undergoing orthodontic treatment with fixed appliances: L.A. Estrada, et al.; Clin. Oral. Investig. 27, 4225 (2023), Sample Type: Human Other (DNA extracts from blood), Abstract
- Long-Term Administration of Vespa velutina nigrithorax Venom Ameliorates Alzheimer’s Phenotypes in 5xFAD Transgenic Mice: Jeong, Y. A., Yun, H. S., et al.; Toxins (Basel) 15, (2023), Sample Type: Mouse Plasma, Abstract
- The Dietary Fermentable Fiber Inulin Alters the Intestinal Microbiome and Improves Chronic Kidney Disease Mineral-Bone Disorder in a Rat Model of CKD: A. Biruete, et al.; bioRxiv , (2023), Sample Type: Rat Serum, Abstract
- Proteomic profiling and ROC analysis identify CD151 and ELAVL1 as potential therapy response markers for the antiviral drug in resistant TNBC: R. Marni, et al.; Life Sci. 320, 121534 (2023), Sample Type: Human Cell Lysate ( MDA-MB 231 and MDA-MB 468 Triple-negative breast cancer cell lines ), Abstract
- Wasp Venom Ameliorates Scopolamine-Induced Learning and Memory Impairment in Mice: J.H. Chae, et al.; Toxins 14, 256 (2022), Sample Type: Mouse Plasma, Abstract
- Cucurbitacin B exerts neuroprotection in a murine Alzheimer’s disease model by modulating oxidative stress, inflammation, and neurotransmitter levels: Liu, Z., Kumar, M., et al.; Front. Biosci. (Landmark Ed.) 27, 71 (2022), Sample Type: Rat Tissue Lysate (Brain), Abstract
- Octanoic Acid – An Insecticidal Metabolite of Conidiobolus coronatus (Entomopthorales) That Affects Two Majors Antifungal Protection Systems in Galleria mellonella (Lepidoptera): Cuticular Lipids and Hemocyte: A. Kaczmarek, et al.; Int. J. Mol. Sci. 23, 5204 (2022), Sample Type: Insect (Wax moth [G. mellonella]) Other (Hemolymph), Abstract
- Arbutin protects brain against middle cerebral artery occlusion-reperfusion (MCAo/R) injury: M. Kumar, et al.; Biochem. Biophys. Res. Commun. 577, 52 (2021), Sample Type: Mouse Tissue Lysate (Brain), Abstract
- The Mechanisms of Cucurbitacin E as a Neuroprotective and Memory-Enhancing Agent in a Cerebral Hypoperfusion Rat Model: Attenuation of Oxidative Stress, Inflammation, and Excitotoxicity: Liu, Z., Kumar, M., et al.; Front. Pharmacol. 12, 794933 (2021), Sample Type: Rat Tissue Lysate (Brain), Abstract
- Accelerated senescence as a cost of reproduction: Testing associations between oxidative stress and reproductive effort in rural and urban women: Sancilio, A., Jasieńska, G., et al.; Am. J. Hum. Biol. 33, e23537 (2021), Sample Type: Human Urine, Abstract
- Death-associated protein kinase 1 correlates with podocyte apoptosis and renal damage and can be mediated by miR-361: Wu, G. J., Zhao, H. B., et al.; Histol. Histopathol. 36, 1155 (2021), Sample Type: Mouse Culture Supernatant (Renal podocytes), Abstract
- Berberine ameliorates renal injury in a rat model of D-galactose-induced aging through a PTEN/Akt-dependent mechanism: H.E. El-Horany, et al.; Arch. Physiol. Biochem. 126, 157 (2020), Sample Type: Rat Serum, Abstract
- Evaluation of Effectiveness of Ultraviolet Emitting Lamps on the Cutaneous Production of Vitamin D3: Relationship of the Lamps Vitamin D3 Producing Potential to the Production of 8-Hydroxy-2′-Deoxyguanosine and Nitric Oxide: A. Shirvani, et al.; Anticancer Res. 40, 565 (2020), Sample Type: Human Tissue Lysate (Skin), Abstract
- In Vivo Anti-Inflammatory Potential of Viscozyme®-Treated Jujube Fruit: Y. Kim, et al.; Foods 9, 1033 (2020), Sample Type: Mouse Tissue Lysate (Liver), Abstract — Full Text
- N-acetylcysteine (NAC), an anti-oxidant, does not improve bone mechanical properties in a rat model of progressive chronic kidney disease-mineral bone disorder: M.R. Allen, et al.; PLoS One 15, e0230379 (2020), Sample Type: Rat Plasma, Abstract — Full Text
- Fumonisin B1 Epigenetically Regulates PTEN Expression and Modulates DNA Damage Checkpoint Regulation in HepG2 Liver Cells: Arumugam, T., Ghazi, T., et al.; Toxins (Basel) 12, (2020), Sample Type: Human Other (DNA extracts of HepG2 cells), Abstract
- Leukocyte Telomere Length, DNA Oxidation, and Risk of Lower-Extremity Amputation in Patients With Long-standing Type 1 Diabetes: Sanchez, M., Hoang, S., et al.; Diabetes Care 43, 828 (2020), Sample Type: Human Plasma, Abstract
- Inhibitory Effect of Steamed Soybean Wastewater Against DSS-Induced Intestinal Inflammation in Mice: S. Jeong, et al.; Foods 9, 954 (2020), Sample Type: Mouse Plasma, Abstract — Full Text
- Oxidative stress as a hidden cost of attractiveness in postmenopausal women: Marcinkowska, U. M., Ziomkiewicz, A., et al.; Sci. Rep. 10, 21970 (2020), Sample Type: Human Urine, Abstract
- Neuroprotective Effects of Euonymus alatus Extract on Scopolamine-Induced Memory Deficits in Mice: Y. Woo, et al.; Antioxidants (Basel) 9, 449 (2020), Sample Type: Mouse Serum, Abstract — Full Text
- The use of a bed with an insulating system of electromagnetic fields improves immune function, redox and inflammatory states, and decrease the rate of aging: Díaz-Del Cerro, E., Vida, C., et al.; Environ. Health 19, 118 (2020), Sample Type: Human Cell Lysate (Blood cells), Abstract
- Circulating uromodulin inhibits systemic oxidative stress by inactivating the TRPM2 channel: LaFavers, K., Macedo, E., et al.; Sci. Transl. Med. 11, (2019), Sample Type: Mouse Tissue Lysate (Lung, kidney ), Abstract
- Antioxidative effects of olive polyphenols compared to vitamin E in piglets fed a diet rich in N-3 PUFA: J. Leskovec, et al.; Animals (Basel) 9, 161 (2019), Sample Type: Pig Serum, Abstract — Full Text
- MicroRNA-204-3p Attenuates High Glucose-Induced MPC5 Podocytes Apoptosis by Targeting Braykinin B2 Receptor: X. Han, et al.; Exp. Clin. Endocrinol. Diabetes 127, 387 (2019), Sample Type: Mouse Cell Lysate (MCP5 podocytes), Abstract
- Oxidative stress and mitochondrial responses to stress exposure suggest that king penguins are naturally equipped to resist stress: A. Stier, et al.; Sci. Rep. 9, 8545 (2019), Sample Type: Bird (Penguin) Plasma and Cell Lysate (Erythrocytes), Abstract — Full Text
- Mangiferin induces radiosensitization in glioblastoma cells by inhibiting nonhomologous end joining: F. Mu, et al.; Oncol. Rep. 40, 3663 (2018), Sample Type: Human Cell Lysate (U118 MG glioblastoma cells), Abstract
- Fibroblast growth factor 23 does not directly influence skeletal muscle cell proliferation and differentiation or ex vivo muscle contractility: K.G. Avin, et al.; Am. J. Physiol. Endocrinol. Metab. 315, E594 (2018), Sample Type: Mouse Culture Supernatant (C2C12 Myoblasts), Abstract
- Nuclear GSK3β induces DNA double-strand break repair by phosphorylating 53BP1 in glioblastoma: Y. Yang, et al.; Int. J. Oncol. 52, 709 (2018), Sample Type: Human Cell Lysate (U87 glioblastoma cells), Abstract — Full Text
- Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses: Z. Zhang, et al.; Mol. Med. Rep. 17, 7142 (2018), Sample Type: Mouse Plasma, Abstract — Full Text
- Plasma concentrations of 8-hydroxy-2′-deoxyguanosine and risk of kidney disease and death in individuals with type 1 diabetes: M. Sanchez, et al.; Diabetologia 61, 977 (2018), Sample Type: Mouse Plasma, Abstract
- The cost of reproduction in women: reproductive effort and oxidative stress in premenopausal and postmenopausal American women: A. Ziomkiewicz, et al.; Am. J. Hum. Biol. 30, e23069 (2018), Sample Type: Human Serum, Abstract
- Proteomic Screen of Brain Glycoproteome Reveals Prion Specific Marker of Pathogenesis: L. Lamoureux, et al.; Proteomics 18, (2018), Sample Type: Mouse Urine, Abstract
- Protective Effects of Dioscorea batatas Flesh and Peel Extracts against Ethanol-Induced Gastric Ulcer in Mice: S. Byeon, et al.; Nutrients 10, 1680 (2018), Sample Type: Mouse Plasma, Abstract — Full Text
- Effects of Valproate Monotherapy on the Oxidant-Antioxidant Status in Mexican Epileptic Children: A Longitudinal Study: E. Beltrán-Sarmiento, et al.; Oxid. Med. Cell. Longev. 2018, 7954371 (2018), Sample Type: Human Plasma, Abstract — Full Text
- miR-370 promotes high glucose-induced podocyte injuries by inhibiting angiotensin II type 1 receptor-associated protein: Y. Xian, et al.; Cell Biol. Int. 42, 1545 (2018), Sample Type: Mouse Cell Lysate (MCP5 podocytes), Abstract
- Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses: Z. Jiang, et al.; Mol. Med. Rep. 16, 5219 (2017), Sample Type: Human Cell Lysate, Abstract — Full Text
- Protective effect of glyceollins in a mouse model of dextran sulfate sodium-induced colitis: H. Seo, et al.; J. Med. Food. 20, 1055 (2017), Sample Type: Mouse Plasma, Abstract
- Environmental conditions can modulate the links among oxidative stress, age, and longevity: V. Marasco, et al.; Mech. Ageing. Dev. 164, 100 (2017), Sample Type: Bird (Zebra finch [Taeniopygia guttata]) Plasma, Abstract
- Effects of Zinc Supplementation on DNA Damage in Rats with Experimental Kidney Deficiency: S.Ç. Yegin, et al.; Biol. Trace Elem. Res. 176, 338 (2017), Sample Type: Rat Serum, Abstract
- The Effect of Thymoquinone on Nuclear Factor Kappa B Levels and Oxidative DNA Damage on Experimental Diabetic Rats: A. Usta & S. Dede; Pharmacogn Mag 13, 458 (2017), Sample Type: Rat Serum, Abstract — Full Text
- Joint association of fruit and heterocyclic amine intake with DNA damage levels in a general population: A.M. de Carvalho, et al.; Nutrition 32, 260 (2016), Sample Type: Human Plasma, Abstract
- The Dose-Dependent Organ-Specific Effects of a Dipeptidyl Peptidase-4 Inhibitor on Cardiovascular Complications in a Model of Type 2 Diabetes: J.Y. Moon, et al.; PLoS One 11, e0150745 (2016), Sample Type: Mouse Urine, Abstract — Full Text
- Effects of Moderate Aerobic Exercise on Cognitive Abilities and Redox State Biomarkers in Older Adults: A.H. Alghadir, et al.; Oxid. Med. Cell. Longev. 2016, Article ID 2545168 (2016), Sample Type: Human Serum, Abstract — Full Text
- Investigation of the effect of the efficiency of noise at different intensities on the DNA of the newborns: Ceylan, N., Kaba, S., et al.; Noise Health 18, 7 (2016), Sample Type: Rat Serum, Abstract
- Skeletal Muscle Regeneration and Oxidative Stress Are Altered in Chronic Kidney Disease: K.G. Avin, et al.; PLoS One 11, e0159411 (2016), Sample Type: Rat Plasma, Abstract — Full Text
- Evidence for the cost of reproduction in humans: high lifetime reproductive effort is associated with greater oxidative stress in post-menopausal women: A. Ziomkiewicz, et al.; PLoS One. 11, e0145753 (2016), Sample Type: Human Urine, Abstract
- Panax ginseng Fraction F3 Extracted by Supercritical Carbon Dioxide Protects against Oxidative Stress in ARPE-19 Cells: C.C. Yang, et al.; Int. J. Mol. Sci. 17, e1717 (2016), Sample Type: Human Cell Lysate (ARPE-19 pigmented retina epithelium cells), Abstract — Full Text
- Serum levels of 8-hydroxy-2′-deoxyguanosine DNA in patients with breast cancer and endometrial cancer with and without diabetes mellitus: L.M. Berstein, et al.; Bull. Exp. Biol. Med. 161, 547 (2016), Sample Type: Human Serum, Abstract
- Oxidative stress and antioxidant responses to progressive resistance exercise intensity in trained and untrained males: H. Çakır-Atabek, et al.; Biol. Sport 32, 321 (2015), Sample Type: Human Serum, Abstract — Full Text
- Epigenetic Study of Parkinson’s Disease in Experimental Animal Model: A.F. Salama, et al.; Int. J. Clin. Exp. Neurology 3, 11 (2015), Sample Type: Rat Serum, Full Text
- Protective effects of alpha-lipoic acid against benzene induced toxicity in experimental rats: M.M. Batsh, et al.; Eur. Rev. Med. Pharmacol. Sci. 19, 2717 (2015), Sample Type: Rat Serum, Abstract — Full Text
- Neuroprotective Role of Vitamin B3 in Experimentally Induced Oxidative Stress: A.E. Atrash, et al.; Int. J. Clin. Exp. Neurology 3, 21 (2015), Sample Type: Rat Serum, Full Text
- Association of oxidative stress biomarkers with adiposity and clinical staging in women with breast cancer: A.A. Carioca, et al.; Eur. J. Clin. Nutr. 69, 1256 (2015), Sample Type: Human Plasma, Abstract
- Arterial stiffness, oxidative stress, and smoke exposure in wildland firefighters: D. Gaughan, et al.; Am. J. Ind. Med. 57, 748 (2014), Sample Type: Human Urine, Abstract
- Genetic damage in patients with chronic kidney disease, peritoneal dialysis and haemodialysis: a comparative study: A. Rangel-López, et al.; Mutagenesis 28, 219 (2013), Sample Type: Human Serum, Abstract — Full Text
- The antioxidant activity of copper (II)(3, 5-diisopropyl salicylate) 4 and its protective effect against streptozotocin-induced diabetes mellitus in rats: M. Qazzaz, et al.; Biol. Trace. Elem. Res. 154, 88 (2013), Sample Type: Rat Serum, Abstract
- Discovery and characterization of artifactual mutations in deep coverage targeted capture sequencing data due to oxidative DNA damage during sample preparation: M. Costello, et al.; Nucleic Acids Res. 41, e67 (2013), Sample Type: Other (DNA extracts), Abstract — Full Text
- Evaluation of sperm DNA peroxidation in fertile and subfertile dogs: B.V. Lopes-Santiago, et al.; Reprod. Domest. Anim. 47, 208 (2012), Sample Type: Canine (Dog) Other (Seminal fluid), Abstract
- Increased oxidative DNA damage and impaired antioxidant defense system in patients with gastrointestinal cancer: A. Gönenç, et al.; Eur. J. Intern. Med. 23, 350 (2012), Sample Type: Human Serum, Abstract
- Cell therapy with human renal cell cultures containing erythropoietin-positive cells improves chronic kidney injury: L.M. Yamaleyeva, et al.; Stem Cells Transl. Med. 1, 373 (2012), Sample Type: Rat Urine, Abstract — Full Text
- Radiation exposure differentially affects songbird 8-hydroxy-2′-deoxyguanosine plasma profiles: ionizing radiation damage response in songbirds: T.W. Luloff, et al.; Environ. Mol. Mutagen. 52, 658 (2011), Sample Type: Bird Plasma, Abstract
- Contrary effects of the receptor tyrosine kinase inhibitor vandetanib on constitutive and flow-stimulated nitric oxide elaboration in humans: E.L. Mayer, et al.; Hypertension 58, 85 (2011), Sample Type: Human Plasma, Abstract — Full Text
- Lyophilized plasma reconstituted with ascorbic acid suppresses inflammation and oxidative DNA damage: P.Y. Van, et al.; J. Trauma 71, 20 (2011), Sample Type: Pig Serum, Abstract
- Comparison of the influence of four classes of HIV antiretrovirals on adipogenic differentiation: the minimal effect of raltegravir and atazanavir: R. Minami, et al.; J. Infect. Chemother. 17, 183 (2011), Sample Type: Culture Supernatant, Abstract
- In vivo antioxidant potential of Sweet chestnut (Castanea sativa Mill.) wood extract in young growing pigs exposed to n-3 PUFA-induced oxidative stress: T. Frankic, J. Salobir; J. Sci. Food. Agric. 91, 1432 (2011), Sample Type: Pig Urine, Abstract
- The impact of OGG1, MTH1 and MnSOD gene polymorphisms on 8-hydroxy-2′-deoxyguanosine and cellular superoxide dismutase activity in myocardial ischemia–reperfusion: B. Karahalil, et al.; Mol. Biol. Rep. 38, 2427 (2011), Sample Type: Human Serum, Abstract
- Antimutagenic activity of mitochondria-targeted plastoquinone derivative: V.A. Chistyakov, et al.; Biochemistry (Mosc) 75, 269 (2010), Sample Type: Rat Serum, Abstract — Full Text
- MAT1A variants are associated with hypertension, stroke, and markers of DNA damage and are modulated by plasma vitamin B-6 and folate: C.Q. Lai, et al.; Am. J. Clin. Nutr. 91, 1377 (2010), Sample Type: Human Urine, Abstract — Full Text
- Antioxidant enzyme activities and DNA damage in children with type 1 diabetes mellitus after 12 weeks of exercise: J. Woo, et al.; Acta Paediatr. 99, 1263 (2010), Sample Type: Human Serum, Abstract
- Altered salivary redox homeostasis in patients with systemic sclerosis: H. Su, et al.; J. Rheumatol. 37, 1858 (2010), Sample Type: Human Saliva, Abstract
- A radiation-induced adaptive response prolongs the survival of prion-infected mice: M. Plews, et al.; Free. Radic. Biol. Med. 49, 1417 (2010), Sample Type: Mouse Urine, Abstract
- Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study: J. Shen, et al.; Am. J. Clin. Nutr. 91, 337 (2010), Sample Type: Human Urine, Abstract — Full Text
- The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs: T. Frankic, et al.; Animal 4, 572 (2010), Sample Type: Pig Urine, Abstract — Full Text
- The teratogenicity and behavioral teratogenicity of di(2-ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) in a chick model: S. Abdul-Ghani, et al.; Neurotoxicol. Teratol. 34, 56 (2010), Sample Type: Bird (Chicken) Serum, Abstract
- Ultraviolet A regulates adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells via up-regulation of Kruppel-like factor 2: J. Lee, et al.; J. Biol. Chem. 285, 32467 (2010), Sample Type: Cell Lysate, Abstract — Full Text
- Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication: H.W. Gil, et al.; Clin. Toxicol. (Phila) 47, 870 (2009), Sample Type: Human Urine, Abstract
- The comparison of in vivo antigenotoxic and antioxidativecapacity of two propylene glycol extracts of Calendulaofficinalis (marigold) and vitamin E in young growing pigs: T. Frankic, et al.; J. Anim. Physiol. Anim. Nutr. (Berl.) 93, 688 (2009), Sample Type: Pig Urine, Abstract
- Salivary DNA, lipid, and protein oxidation in nonsmokers with periodontal disease: H. Su, et al.; Free Radic. Biol. Med. 46, 914 (2009), Sample Type: Human Saliva, Abstract
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Last modified: May 29, 2024
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