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Caspase-3 polyclonal antibody

ADI-AAP-113

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The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.

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This antibody is covered by our Worry-Free Guarantee.

Caspase-3 polyclonal antibody Immunohistochemistry — Immunohistochemistry analysis of human spleen tissue stained with Caspase-3, pAb at 10µg/ml.

Caspase-3 polyclonal antibody Western blot — Western blot analysis of Caspase-3 pAb: Lane 1: MW Marker, Lane 2: Jurkat (Prod. No. ADI-LYC-JK100), Lane 3: Jurkat treated with staurosporine, Lane 4: MCF-7 (negative control), Lane 5: HeLa, (cell lysate) (Prod. No. ADI-LYC-HL100), Lane 6: Rat Spleen, Lane 7: Mouse Spleen.

ADI-AAP-113 Caspase-3 polyclonal antibody Western Blot Human 1 — Eupafolin sensitizes TRAIL-induced apoptosis in Caki cellsA-C. Caki cells were treated with 50 ng/ml TRAIL in the presence or absence of the indicated concentrations with eupafolin for 24 h (A) or indicated time periods (B and C). Apoptosis was analyzed as a sub-G1 population by flow cytometer. The protein levels of PARP and actin were determined by Western blotting. The level of actin was used as a loading control. D-F. Caki cells were treated with 50 ng/ml TRAIL in the presence or absence of 30 μM eupafolin for 24 h. Cell morphology was detected by interference light microscopy (D). The condensation and fragmentation of the nuclei were detected by 4′, 6′-diamidino-2-phenylindole staining (E). The DNA fragmentation detection kit determined the fragmented DNA (F). G. Isoboles were obtained by plotting the combined concentrations of each drug required to produce 50% cell death. The straight line connecting the IC50 values obtained for the two agents when applied alone corresponded to the addition of their independent effects. Values below this line indicate synergy, whereas values above this line indicate antagonism. H. Caki cells were treated with 50 ng/ml TRAIL in the presence or absence of 30 μM eupafolin for 24 h. Caspase activities were determined with colorimetric assays using caspase-3 DEVDase assay kits. I. Caki cells were treated with 30 μM eupafolin plus 50 ng/ml TRAIL for 24 h in the presence or absence of 20 μM z-VAD-fmk (z-VAD). The sub-G1 fraction was measured by flow cytometry. The protein expression levels of PARP, caspase-3, cleaved caspase-3 and actin were determined by Western blotting. The level of actin was used as a loading control. J. Caki cells were treated with 30 μM eupafolin for the indicated time periods and loaded with a rhodamine123 fluorescent dye. The mitochondrial membrane potential (MMP) was measured using a flow cytometer. K. Caki cells were treated with 50 ng/ml TRAIL in the presence or the absence of 30 μM eupafolin for 24 h. Cytoplasmic fractions were analyzed for cytochrome c release. The level of COX IV was used as a mitochondria loading control. The level of actin was used as a loading control. Apoptosis was analyzed as a sub-G1 population by FACS. The values in (A, B, F, H, I and J) represent the mean ± SD from three independent samples. * p < 0.05 compared to the control. # p < 0.01 compared to the combined treatment with eupafolin and TRAIL.

Image collected and cropped by CiteAb under a CC-BY license from the following publication: Eupafolin enhances TRAIL-mediated apoptosis through cathepsin S-induced down-regulation of Mcl-1 expression and AMPK-mediated Bim up-regulation in renal carcinoma Caki cells. Oncotarget (2016)

Product Details

Application	IHC (PS), WB
Application Notes	Detects a band of ~36kDa by Western blot. A lower molecular weight band refers to a proteolytic cleavage product of caspase-3.
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
GenBank ID	U26943
Host	Rabbit
Immunogen	Recombinant human Caspase-3 protein (full length).
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Yeast
UniProt ID	P42574
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Selenite selectively kills lung fibroblasts to treat bleomycin-induced pulmonary fibrosis.: Lin, J. H., Liu, C. C., et al.; Redox Biol. 72, 103148 (2024), Application(s): WB, Abstract
Akt enhances the vulnerability of cancer cells to VCP/p97 inhibition-mediated paraptosis: Lee, D. M., Kim, I. Y., et al.; Cell Death Dis. 15, 48 (2024), Abstract
ITC-6102RO, a novel B7-H3 antibody-drug conjugate, exhibits potent therapeutic effects against B7-H3 expressing solid tumors: Shin, S. H., Ju, E. J., et al.; Cancer Cell Int. 23, 172 (2023), Application(s): WB, Abstract
Structure-activity relationships reveal a 2-furoyloxychalcone as a potent cytotoxic and apoptosis inducer for human U-937 and HL-60 leukaemia cells: Del Rosario, H., Saavedra, E., et al.; Bioorg. Chem. 127, 105926 (2022), Abstract
Dapagliflozin induces apoptosis by downregulating cFILPL and increasing cFILPS instability in Caki-1 cells.: Jang, J. H., Lee, T. J., et al.; Oncol. Lett. 24, 401 (2022), Application(s): WB, Abstract
Impaired Cdc20 signaling promotes senescence in normal cells and apoptosis in non-small cell lung cancer cells: Volonte, D., Sedorovitz, M., et al.; J. Biol. Chem. 298, 102405 (2022), Abstract
Lucanthone, Autophagy Inhibitor, Enhances the Apoptotic Effects of TRAIL through miR-216a-5p-Mediated DR5 Upregulation and DUB3-Mediated Mcl-1 Downregulation: J.Y. Yoon, et al.; Int. J. Mol. Sci. 23, 17 (2022), Abstract
Lactucin induces apoptosis through reactive oxygen species-mediated BCL-2 and CFLARL downregulation in Caki-1 cells: J.H. Jang, et al.; Genes Genomics 43, 1199 (2021), Abstract
Apoptosis Pathways Triggered by a Potent Antiproliferative Hybrid Chalcone on Human Melanoma Cells.: Quintana, J., Estévez, F., et al.; Int. J. Mol. Sci. 22, (2021), Application(s): WB / Reactant(s): Human, Abstract
Pioglitazone mediates apoptosis in Caki cells via downregulating c-FLIP(L) expression and reducing Bcl-2 protein stability.: Kim, J. Y., Jang, J. H., et al.; Oncol. Lett. 22, 743 (2021), Application(s): WB, Abstract