Nitric Oxide (total), detection kit

• Efficient – only 50 µl diluted sample needed, saving your precious sample
• Sensitive – detect as little as 0.625 µmol/L
• Trusted – widely cited in peer-reviewed publications
• Fast – results from up to 80 samples in duplicate in just 40 minutes

The Nitric Oxide (total), detection kit is based on the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase, followed by the Griess reaction to form a colored azo dye product. Quantification is performed by measuring absorption at 540-570 nm. Only 0.050 ml diluted sample needed per well saves precious sample.

Nitric oxide (NO) is a major mammalian secretory product that initiates host defense, homeostatic and developmental functions by either direct effect or intercellular signaling. NO is the product of a five-electron oxidation of the amino acid L-arginine mediated by nitric oxide synthase. As a direct effector, NO is thought to activate regulatory proteins, kinases and proteases that are directed by reactive oxygen intermediates. Activation of the immune system is associated with an increase in macrophage NO production. Soluble NO exerts a variety of homeostatic influences as an activator of soluble guanylyl cyclase, a neuronal potentiator, a peripheral nervous system neurotransmitter, and a contraction regulator of both smooth muscle and vascular tissue. In addition, NO has been linked to the formation of olfactory and synaptic memories and remodeling.