Nitric Oxide (NO₂^–/NO₃^–) detection kit

• Homogeneous mix and read assay
• Measures nitrate and nitrite separately
• Fast results in 10 to 40 minutes from up to 39 samples in duplicate

The Nitric Oxide (NO₂^–/NO₃^–), detection kit is a complete kit for the quantitative determination of nitrate and nitrite concentrations. Absorbance is read at 540-570 nm. Only 0.050 ml diluted sample needed per well saves precious sample.

Nitric oxide (NO) is a major mammalian secretory product that initiates host defense, homeostatic and developmental functions by either direct effect or intercellular signaling. NO is the product of a five-electron oxidation of the amino acid L-arginine mediated by nitric oxide synthase. As a direct effector, NO is thought to activate regulatory proteins, kinases and proteases that are directed by reactive oxygen intermediates. Activation of the immune system is associated with an increase in macrophage NO production. Soluble NO exerts a variety of homeostatic influences as an activator of soluble guanylyl cyclase, a neuronal potentiator, a peripheral nervous system neurotransmitter, and a contraction regulator of both smooth muscle and vascular tissue. In addition, NO has been linked to the formation of olfactory and synaptic memories and remodeling.