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17β-Estradiol ELISA kit

ADI-901-008

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Highly sensitive ELISA, measuring as little as 28.5 pg/ml of 17β-Estradiol in < 3 hours

Specific with low cross-reactivity to related steroids
Safe and non-radioactive enzymatic assay
Simple and color-coded reagents reduces error
Reliable and reproducible results day-after-day and lot-after-lot
Fully quantitative results that surpass semi-quantitative Western blot analysis
Save with bulk kit package!

The 17β-Estradiol ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This kit offers specific and save non-radioactive quantification of estradiol with ready-to-use liquid color-coded reagents to reduce error.

Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.

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Product Details

Alternative Name	Estrogen
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of 17β-Estradiol in culture supernatants and saliva from any species. For serum and plasma use ADI-901-174.
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
Sensitivity	28.5 pg/ml (range 29.3 – 30,000 pg/ml)
Species Reactivity	Species independent
Technical Info / Product Notes	Cited samples: Hormone ELISAs: Cited Samples Wildlife Endocrinology: Cited Sample Types
Wavelength	405 nm

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Differential Effects of Canonical Androgens and 11-Ketotestosterone on Reproductive Phenotypes and Folliculogenesis in Mouse Model of PCOS: Tsai, Y. R., Liao, Y. N., et al.; Biomedicines 13, 1077 (2025), Abstract
ADAMTS-1, a multifunctional proteinase, in the uterus of both estrous cycle rats and ovariectomized rats can be regulated via hormones: Parlak Ak, T., Yaman, M., et al.; Histochem. Cell Biol. 163, 62 (2025), Abstract
Personalized mixture toxicity testing: A proof-of-principle in vitro study evaluating the steroidogenic effects of reconstructed contaminant mixtures measured in blood of individual adults: Strand, D., Lundgren, B., et al.; Environ. Int. 192, 108991 (2024), Abstract
Screening persistent organic pollutants for effects on testosterone and estrogen synthesis at human-relevant concentrations using H295R cells in 96-well plates: Strand, D., Nylander, E., et al.; Cell Biol. Toxicol. 40, 69 (2024), Abstract
Menstrual phase influences cerebrovascular responsiveness in females but may not affect sex differences: Skinner, B. D., Weaver, S. R. C., et al.; Front. Physiol. 13, 1035452 (2023), Abstract
Pyometra alters the redox status and expression of estrogen and progesterone receptors in the uterus of domestic cats: A.E.J. Nascimento, et al.; J. Feline Med. Surg. 25, 1098612X231170159 (2023), Abstract
Improvement of Estrogen Deficiency Symptoms by the Intake of Long-Term Fermented Soybeans (Doenjang) Rich in Bacillus Species through Modulating Gut Microbiota in Estrogen-Deficient Rats: T. Zhang, et al.; Foods 12, 1143 (2023), Abstract
Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022), Abstract
Aggressive but not reproductive boldness in male green anole lizards correlates with baseline vasopressin activity: Kabelik, D., Julien, A. R., et al.; Horm. Behav. 140, 105109 (2022), Abstract
Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021), Abstract