Serotonin ELISA kit

• Highly sensitive measurement of serotonin, detecting as little as 0.293 ng/ml
• No acylation required for standards and samples
• Thoroughly validated in multiple complex sample matrices
• High throughput format with results in just 3 hours for up to 39 samples in duplicate
• Fully quantitative results that surpass semi-quantitiave Western blot analysis

The Serotonin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours.

Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions in neurotransmission, gastric motility, hemostasis, and cardiovascular integrity. Seven subclasses (5-HT 1-7) of serotonin receptors have been identified, a majority of which are members of the seven transmembrane domain-containing GPCR family, and are the target of several anti-depressant drugs.  The 5-HT1 subclass consists of five highly homologous receptors that couple via G(i/o) alpha subunits to inhibit adenylyl cyclase activity. 5-HT1A expression in mammals is strongest in CNS limbic pathways that control emotion, including tissue of the hippocampus, dorsal raphé, and septum. Serotonin is a key biomarker used for the monitoring of overall welfare and/or the assessment of behavioral disorders in a variety of species.