8-iso-PGF_2α ELISA kit

• Fast – results from up to 36 samples in duplicate in less than 3 hours
• Ready-to-use – liquid color-coded reagents and pre-coated plates reduce errors
• Trusted – published in peer-reviewed journals
• Reliable – reproducible results day-after-day and lot-after-lot

The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. This is a colorimetric competitive immunoassay kit for the quantitative determination of free 8-iso-Prostaglandin F_2α in biological fluids such as urine and in culture supernatants. To measure 8-iso-Prostaglandin F_2α levels in serum, plasma or other tissue samples, use the Direct 8-iso-PGF_2α EIA Kit.

8-iso-PGF_2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF_2α has been shown to circulate in plasma and is excreted in urine.

Prostaglandin F_2α (PGF_2α) is formed in a variety of cells from PGH₂, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF_2α is often viewed as an antagonist to PGE₂ due to their opposing effects on various tissues. PGF_2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF_2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF_2α have also been associated with preeclampsia.