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ELISA kits with AMP’D® Technology
Quantify Difficult-to-Detect Analytes
Enzo’s AMP’D® Technology
Increase ELISA Sensitivity with the Signal Amplification AMP’D® Technology
The AMP’D® ELISA Signal Amplification system is designed to replace traditional alkaline phosphatase (AP) substrates, such as pNPP (p-Nitrophenyl phosphate), with a combination substrate and amplifier system that results in greater sensitivity when compared to a traditional substrate ELISA.
In a conventional detection system, enzyme bound to the microtiter plate interacts directly with the substrate producing a color change where the resulting absorbance is directly proportional to the amount of captured analyte. In the Amp’d ELISA system, bound AP converts a substrate that is utilized in a second enzyme reaction system which is initiated by addition of the amplifier reagent. It is this amplification step that allows for greater (amplified) color production at lower analyte concentrations resulting in an increase in assay sensitivity.
In the Amp’d ELISA system, the critical substrate component, NADPH, is added to wells containing AP, and the AP reduces to NADH via release of a phosphate group. This reaction is allowed to proceed for an amount of time with the accumulated NADH being proportional to the amount of analyte/bound AP-conjugate. Upon the addition of the reconstituted amplifier reagent, this first reaction is quenched and the NADH feeds a second redox enzyme system. Here diaphorase utilizes NADH to reduce the iodonitrotetrazolium salt into formazan (purple color) producing NAD+. A counter enzymatic reaction then occurs where the NAD+ is reduced to NADH while ethanol is oxidized to acetaldehyde via alcohol dehydrogenase. These enzymatic reactions proceed for a period of time, recycling the NADH thus amplifying the original AP/substrate reaction. The resulting color intensity is ultimately proportional to the amount of bound analyte.
AMP’D® ELISA Kits
AMP’D® ELISA Signal Amplification Kit
Our AMP’D® ELISA kit provides increase in sensitivity over traditional ELISAs while detecting lower concentrations of target in samples
- Increase sensitivity up to 50-fold over traditional ELISA
- Quantify difficult-to-detect analytes
- Minimal additional time needed to complete amplification step
- Flexible format for use with any immunometric, sandwich ELISAs where greater sensitivity is desired
- Convenient one or five 96-well plate sizes for high throughput analysis
- Easy-to-use, simple procedure with nominal ELISA protocol modification
Enhanced Signal Over Traditional ELISAs
Our Featured Products
AMP’D® HSP70 high sensitivity ELISA kit
Ultra-sensitive (7 pg/ml) AMP’D® HSP70 high sensitivity ELISA kit enabling the ability to use less sample and detect both baseline and upregulated levels of human, mouse and rat Hsp70 (Hsp72), a major chaperone, cancer biomarker, and key cell stress regulator.
Learn more AMP’D® GLP-1 ELISA kit
Ultra-sensitive AMP’D® GLP-1 ELISA kit enabling the ability to use less sample to detect levels of human Glucagon-like peptide 1 (7-36) amide, a potent promoter of insulin secretion, a major incretin hormone, and therapeutic for type 2 diabetes.
Learn moreAMP’D® Certified kit and supporting reagents
The standard Enzo APP ΔC31 ELISA kit can be AMP’D® using an AP-conjugate antibody and our AMP’D™ detection system (AMP’D® ELISA Signal Amplification Kit). Results show up to ~10-fold increase in sensitivity (from 0.92 pM to 0.094 pM) which will allow for more reliable analysis of samples with low concentrations of APP ΔC31. We also offer Goat anti-rabbit IgG, pAb (AP conjugate) and Streptavidin (AP Conjugate) as accessory products for use with AMP’D® Technology.
