DNA Methylation Detection

Colorimetric Assay Based Detection

DNMT Colorimetric Drug Discovery Kit

• Rapid – results in less than 4 hours
• Simple and safe – Easy colorimetric assay without radioactivity or chromatography
• Sensitive – measure as little as 0.5 μg of nuclear extract or 0.5 ng of purified enzymes
• Specific – optimized to allow high specificity to 5-mC without cross-reactivity to unmethylated cytosine
• Reliable – reproducible results

ELISA-Based Detection

5-Methylcytosine DNA ELISA kit

• Accurately quantitate 5-mC in any DNA sample in less than 3 hours
• Ideal for high-throughput analysis
• High specificity comparable to LC-MS/MS-MRM analysis

Accurately Quantify 5-mc DNA

Genomic DNA Mass Spectroscopy versus ELISA analysis: The 5-mC DNA ELISA Kit (prod. no. ADI-900-224) quantifies 5-mC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).