DNA Methylation Detection

Optimal Tools for Detecting DNA Methylation

Colorimetric Assay Based Detection

DNMT Colorimetric Drug Discovery Kit

  • Rapid – results in less than 4 hours
  • Simple and safe – Easy colorimetric assay without radioactivity or chromatography
  • Sensitive – measure as little as 0.5 μg of nuclear extract or 0.5 ng of purified enzymes
  • Specific – optimized to allow high specificity to 5-mC without cross-reactivity to unmethylated cytosine
  • Reliable – reproducible results
Product Information

ELISA-Based Detection

5-Methylcytosine DNA ELISA kit

  • Accurately quantitate 5-mC in any DNA sample in less than 3 hours
  • Ideal for high-throughput analysis
  • High specificity comparable to LC-MS/MS-MRM analysis
Accurately Quantify 5-mc DNA

Genomic DNA Mass Spectroscopy versus ELISA analysis: The 5-mC DNA ELISA Kit (prod. no. ADI-900-224) quantifies 5-mC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).

Product Information

5-Hydroxymethylcytosine DNA ELISA kit

ADI-900-225

Highly sensitive, rapid ELISA kit to quantify 5-hydroxymethylcytosine in DNA with results that closely correlate to mass spectrometry analysis.

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ELISA kit
Application Colorimetric detection, ELISA
Assay Time ~3 hours
Sensitivity <0.02% 5-hydroxymethylcytosine DNA per 100ng input DNA (range 0.03-0.55% (100 ng/µl))

Universal methylated DNA standard (mouse)

ENZ-45006

Methylation standard (mouse) to help determine efficiency of a bisulfite DNA conversion

DNMT Colorimetric Drug Discovery Kit

ENZ-45017

Rapid, sensitive, and simple colorimetric DNMT activity/inhibition kit.

Application Colorimetric detection
Assay Time <4 hours

5-Hydroxymethylcytosine polyclonal antibody

ADI-905-904

Alternative Name 5-hmC
Application ELISA, Immunoblot, IP
Host Rabbit
Isotype IgG1
Species Reactivity Species independent