DNA Sample Conversion

Comprehensive tools for high-efficiency conversion

High-efficiency Conversion of 5-mC and 5-hmC DNA for the Truest Downstream Analysis

Conversion allows for resolution of single nucleotide information
Kits provide high conversion rates without the introduction of DNA damage
Converted output sample suitable for detection by bisulfite sequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing

	DNA Only	DNA Only	Blood, Tissue, FFPE, DNA	Blood, Tissue, FFPE, DNA	DNA Only
	Express DNA Methylation Kit	Express DNA Methylation Kit	Blood & Tissue DNA Methylation Kit	Blood & Tissue DNA Methylation Kit	BIOARRAY™ 5-hmC Methylation Kit
Target & Modification	5-mC Bisulfite modification	5-mC Bisulfite modification	5-mC Bisulfite modification	5-mC Bisulfite modification	5-hmC β-Glucosyltransferase protection
Format	Spin Column	96-Well	Spin Column	96-Well	Spin Column
Elution Volume	≥ 10 μl	≥ 15 μl	≥ 10 μl	≥ 15 μl	≥ 6 μl
Conversion Efficiency	>99.5%	>99.5%	>99.5%	>99.5%	*Enzymatic efficiency ~100%
Processing Time	1.5 hours	1.5 hours	4 hours	4 hours	6-8 hours
Input	100 pg – 2 μg of DNA	100 pg – 2 μg of DNA	≥ 50 pg DNA, ≥ 10 cells, blood, tissue, FFPE	≥ 50 pg DNA, ≥ 10 cells, blood, tissue, FFPE	100 ng – 500 ng DNA