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For use in the detection and identification of deubiquitinylating enzymes. HA-Ub-VS is a DUB active site directed probe generated by a chemical ligation method, that acts as a potent and irreversible inhibitor of DUBs through covalent modification of the active site and as a specific probe for enzymes with DUB activity. The HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein, facilitates sensitive identification or purification of HA-Ub-VS modified DUBs through recognition by anti-HA antibodies and/or anti-HA-agarose.
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Image: DUB active site probe assay: Western blot showing reactions containing HAUbVS only (lane 1), HAUbVS + GST-UCHL1 (lane 2, Prod. No. BML-UW9305), and HA-UbVS + USP2cd (lane 3, Prod. No. BML-UW9850). HAUbVS modified proteins detected using HA polyclonal antibody (Sigma – H6908) at 1:1000 dilution.”””””
| Regulatory Status |
RUO – Research Use Only |
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