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Toll-like receptor 7 polyclonal antibody

ALX-210-874

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Toll-like receptor 7 polyclonal antibody Western blot — **Figure 3:** Immunohistochemical analysis of human colon tissue using PAb to Toll-like Receptor 7 (Prod. No. ALX-210-874) and an isotype control (bottom) at 5μg/ml.”””,Toll-like receptor 7 polyclonal antibody Immunohistochemistry,Toll-like receptor 7 polyclonal antibody Immunohistochemistry,yes
ALX-210-874,210-874_1.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-874_1.gif,**Figure 1:** Western blot analysis of TLR7 in Raw cell lysate using Prod. No. ALX-210-874 at 1µg/ml. “””””

Raj — **Figure 2:** Intracellular staining by FACS analysis of TLR7 in Ramos cells (A) and Raw cells (B) using Prod. No. ALX-210-874 at 2µg/ml. Histogram C shows the rabbit IgG isotype control.”””,Toll-like receptor 7 polyclonal antibody Flow Cytometry,Toll-like receptor 7 polyclonal antibody Flow Cytometry,yes
ALX-210-898,210-898.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-898.gif,**Figure:** Western blot comparing three different PAbs to VASP using 25µg human platelet protein. **Lane 1:** PAb to VASP (human) (Prod. N. ALX-210-898). **Lane 2:** PAb to VASP (M4) (Prod. N. ALX-210-725) **(Not available anymore!)**. **Lane 3:** PAb to VASP (affinity purified) (Prod. N. ALX-210-880).,VASP (human) polyclonal antibody Western blot,VASP (human) polyclonal antibody Western blot,yes
ALX-210-904,210-904.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-904.gif,**Figure**: Western blot analysis of human and mouse cell lines with the anti-human NLRP1/NALP1 antibody (AL176) (Prod. No. ALX-210-904).Method: Total protein extracts (20µg) from various human (293-T

Raj — Figure 2: Intracellular staining by FACS analysis of TLR7 in Ramos cells (A) and Raw cells (B) using Prod. No. ALX-210-874 at 2µg/ml. Histogram C shows the rabbit IgG isotype control.”””,Toll-like receptor 7 polyclonal antibody Flow Cytometry,Toll-like receptor 7 polyclonal antibody Flow Cytometry,yes ALX-210-898,210-898.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-898.gif,Figure: Western blot comparing three different PAbs to VASP using 25µg human platelet protein. Lane 1: PAb to VASP (human) (Prod. N. ALX-210-898). Lane 2: PAb to VASP (M4) (Prod. N. ALX-210-725) (Not available anymore!). Lane 3: PAb to VASP (affinity purified) (Prod. N. ALX-210-880).,VASP (human) polyclonal antibody Western blot,VASP (human) polyclonal antibody Western blot,yes ALX-210-904,210-904.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-904.gif,Figure: Western blot analysis of human and mouse cell lines with the anti-human NLRP1/NALP1 antibody (AL176) (Prod. No. ALX-210-904).Method: Total protein extracts (20µg) from various human (293-T

Product Details

Alternative Name	TLR7
Application	Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB
Application Notes	Flow Cytometry: suitable for cell surface and intracellular FC. Western Blot: in human Ramos cells, additional bands are seen
Formulation	Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
Host	Rabbit
Immunogen	Synthetic peptide corresponding to aa 706-728 of human TLR7 (Toll-like receptor 7).
Purity Detail	Protein G-affinity purified.
Recommendation Dilutions/Conditions	Flow Cytometry (intracellular, 0.5-5µg/106 cells)Immunohistochemistry (paraffin sections, 5-10µg/ml)Western Blot (1-3µg/ml)
Species Reactivity	Human, Mouse, Rat
UniProt ID	Q9NYK1
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Use/Stability	Stable for 6 months when stored at +4°C.
Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes.: Pietschmann, T., Gerold, G., et al.; Cells 10, (2021), Application(s): FC/FACS / Reactant(s): Mouse, Abstract
Up-regulation of Toll-like receptors 7 and 9 and its potential implications in the pathogenic mechanisms of LMNA-related myopathies.: Cappelletti, C., Salerno, F., et al.; Nucleus 9, 398 (2018), Application(s): IF / Reactant(s): Human, Abstract
Expression and functionality of Toll- and RIG-like receptors in HepaRG cells: Luangsay, S., Ait-Goughoulte, M., et al.; J. Hepatol. 63, 1077 (2015), Abstract
Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract: M.A. Palladino, et al.; Biol. Reprod. 76, 958 (2007), Abstract
Antitumor effects of imidazoquinolines in urothelial cell carcinoma of the bladder: E.B. Smith, et al.; J. Urol. 177, 2347 (2007), Abstract
The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling: M.M. Brinkmann, et al.; J. Cell Biol. 177, 265 (2007), Abstract — Full Text
Toll-like receptor-7 modulates immune complex glomerulonephritis: R.D. Pawar, et al.; J. Am. Soc. Nephrol. 17, 141 (2006), Abstract — Full Text
Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice: P.S. Patole, et al.; Nephrol. Dial. Transplant. 21, 3062 (2006), Abstract
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line: M.D. Srivastava and B.I. Srivastava; Leuk. Res. 29, 813 (2005), Abstract
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad: S. Francois, et al.; J. Immunol. 174, 3633 (2005), Abstract

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Toll-like receptor 7 polyclonal antibody

ALX-210-874

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