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Rhod-2 AM (ultra pure)

ENZ-52010

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Calcium dye

Rhod-2 AM has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, making it suitable for use with argon and krypton laser excitation sources. Rhod-2 signal intensity one of the strongest of all the calcium probes. Rhod 2 is especially suitable probe for intracellular calcium monitoring by confocal laser-scanning microscopy and flow cytometry. The dissociation constant of Rhod 2 with calcium is among the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Rhod-2 AM is an acetoxymethyl ester derivative of Rhod-2 that can be easily loaded into cells by passive incubation. Wavelength Maxima: Excitation 549nm, Emission 578nm

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Product Details

Alternative Name	1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N’,N’-tetraacetic acid, tetraacetoxy methyl ester . Br
CAS	145037-81-6
MW	1124.0
Purity	≥90% (HPLC)
Solubility	Soluble in DMSO.
Technical Info / Product Notes	This product is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Handling & Storage

Use/Stability	Stable for at least one year after receipt when stored as recommended.
Handling	Protect from light. Keep cool and dry.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Anti-arrhythmic and inotropic effects of empagliflozin following myocardial ischemia: M.A. Azam, eta al.; Life Sci. 276, 119440 (2021), Abstract
The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye: A. Wolf, et al.; Nat. Commun. 11, 2709 (2020), Abstract — Full Text
MICU1 drives glycolysis and chemoresistance in ovarian cancer: P.K. Chakraborty, et al.; Nat. Commun. 8, 14634 (2017), Abstract — Full Text
Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching: C. Du, et al.; Cell Calcium 29, 217 (2001), Abstract
Mitochondrial free calcium levels (Rhod-2 fluorescence) and ultrastructural alterations in neuronally differentiated PC12 cells during ceramide-dependent cell death: M.P. Muriel, et al.; J. Comp. Neurol. 426, 297 (2000), Abstract

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Rhod-2 AM (ultra pure)

ENZ-52010

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

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