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RANK ligand (RANKL), also called TRANCE, OPGL and ODF, is a member of the TNF family that is expressed in activated T cells, lymph nodes and in stromal cell lines. RANKL (TRANCE) interacts with its receptor RANK expressed on mature dendritic cells (DC) and mature osteoclasts, leading to the inhibition of apoptosis, probably through the upregulation of bcl-x. RANKL is, therefore, a useful tool for enhancing DC and osteoclast survival and activity. Osteoprotegerin (OPG) is a potent inhibitor of RANKL.
Historical data has shown that the biological activity of RANKL supports the survival of dendritic cells and osteoclasts.
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Figure 1: Schematic structure of human rhsRANKL (peptide, aa. 151-316).
Figure 2: Binding of rhsRANKL to Osteoprotegerin. Recombinant, human sRANKL binds to Osteoprotegerin:Fc (see Prod. No. ALX-522-007).Method: Ligand binding assay: 96 well ELISA plates were coated O/N with 50 ng OPG-Fc (Prod. No. ALX-522-007) per well. After a blocking step
SDS-PAGE Analysis of RANKL (soluble) (human), (recombinant). Lane 1: MW Marker, Lane 2: 1µg.
Figure 3: Mononuclear cells differentiate into Osteoclasts in the presence of M-CSF and RANKL, Soluble (human) (recombinant) (ALX-522-012). Method: Human CD14+ mononuclear cells isolated from adult peripheral blood were cultured in control medium (upper panel), in 25ng/ml M-CSF (middle panel), or in 25ng/ml M-CSF plus 50ng/ml RANKL (lower panel). Osteoclasts were identified as Tartrate-Resistant Acid Phosphatase (TRAP)-positive multinucleated cells. Osteoclasts were detected exclusively in presence of RANKL and in these culture conditions (M-CSF + RANKL), cells fused and generated multinucleated dark red TRAP-positive cells (arrows). Nuclei were stained with haematoxylin. Original magnification: 200x. Picture courtesy of V.Kindler and D.Suvà, University of Geneva.“””,RANKL (soluble) (human)
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