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Proteasome 26S (human), (purified)

BML-PW9310

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High integrity preparation for use in proteasome research.

Highly purified preparation of ‘26S’ proteasomes useful for carrying out in vitro protein degradation studies with suitably ubiquitinylated protein substrates.

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Proteasome 26S (human), (purified) image — A typical example of a sucrose density gradient separation and purification of 20S, 26S and 30S proteasome derived from human erythrocytes together with electron micrographs of the individual complexes. The protein concentration used for micrography is best at ~2.5µg/mL.

Product Details

Formulation	Suspended in TSD buffer (50μg in 10mM TRIS, containing 25mM potassium chloride, 1.1mM magnesium chloride, 0.1mM ethylenediaminetetraacetic acid, 1mM dithiothreitol, 1mM sodium azide, 2mM ATP, pH 7.0, and 35% glycerol).
Source	Isolated from human erythrocytes. Consists of a high purity mixture of ‘26S’ proteasomes singly (26S) and doubly (30S) capped with 19S regulatory subunit complexes in the ratio of 40% single cap : 60% double capped at the time of preparation.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. When ready for use the enzyme should be thawed by standing on ice. If the enzymatic acitivity of the 26S proteasome is to be measured, it should be used immediately after thawing since the enzyme complex is labile. After dissociaton of the 26S complex the 20S proteasome activity is relatively stable.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

A structure-based designed small molecule depletes hRpn13Pru and a select group of KEN box proteins: X. Lu, et al.; Nat. Commun. 15, 2485 (2024), Abstract
hRpn13 shapes the proteome and transcriptome through epigenetic factors HDAC8, PADI4, and transcription factor NF-kB p50: V. O. Amponsa, et al.; Mol. Cell 84, 522 (2024), Abstract
Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer’s-like pathology in mouse and fly APP overexpression models: E.S. Chocron, et al.; Sci. Adv. 8, abk2252 (2022), Abstract
Proteasome dysfunction in alveolar type 2 epithelial cells is associated with acute respiratory distress syndrome: Sitaraman, S., Na, C. L., et al.; Sci. Rep. 9, 12509 (2019), Application(s): WB, Abstract
Structural Basis for the Inhibitory Effects of Ubistatins in the Ubiquitin-Proteasome Pathway: M.A. Nakasone, et al.; Structure 25, 1839 (2017), Abstract — Full Text
Reaction of complement factors and proteasomes in experimental encephalitis: S. Lange, et al.; J. Neurovirol. 23, 313 (2017), Abstract — Full Text
Dietary apigenin potentiates the inhibitory effect of interferon-α on cancer cell viability through inhibition of 26S proteasome-mediated interferon receptor degradation: S. Li, et al.; Food Nutr. Res. 60, 31288 (2016), Application(s): Cell culture and cell-based assay, Abstract — Full Text
Proteolysis targeting peptide (PROTAP) strategy for protein ubiquitination and degradation: J. Zheng, et al.; Biochem. Biophys. Res. Commun. 470, 936 (2016), Abstract
Identification of nitric oxide as an endogenous inhibitor of 26S proteasomes in vascular endothelial cells: H. Liu, et al.; PLoS One. 9, e98486 (2014), Abstract — Full Text
Reconstitution of hybrid proteasomes from purified PA700-20 S complexes and PA28alphabeta activator: ultrastructure and peptidase activities: F. Kopp et al.; J. Mol. Biol. 313, 465 (2001), Abstract

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Proteasome 26S (human), (purified)

BML-PW9310

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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