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PKA kinase activity kit

ADI-EKS-390A

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Non-radioactive protein kinase assay provides rapid and sensitive detection

Safe and non-radioactive colorimetric measurement of kinase activity
Flexible format, kinetic and end-point options available
Fast results in < 4.5 hours
Efficiency maximized with only 30 µl diluted sample needed per well

The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

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Product Details

Alternative Name	Protein kinase A, cAMP-dependent protein kinase
Application	Activity assay, Colorimetric detection
Application Notes	For the quantitative determination of PKA kinase activity in partially purified, purified, or crude enzyme preparations from any species.
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
Species Reactivity	Species independent
UniProt ID	P17612 (Cα subunit), P22694 (Cβ subunit), P22162 (Cγ subunit), P10644 (RIα subunit), P31321 (RIβ subunit), P13861 (RIIα subunit), P31323 (RIIβ subunit)

Handling & Storage

Use/Stability	Store all components at 4°C, except active kinase at -80°C.
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Activation of hepatic adenosine A1 receptor ameliorates MASH via inhibiting SREBPs maturation: Zhu, W., Hong, Y., et al.; Cell Rep. Med. 5, 101477 (2024), Abstract
Role of intraluteal and intrauterine prostaglandin signaling in LH-induced luteolysis in pregnant rats: Vashistha, A., Khan, H. R., et al.; Mol. Reprod. Dev. 90, 260 (2023), Abstract
Immobilization of azide-functionalized proteins to micro- and nanoparticles directly from cell lysate: Saini, G., Parasa, M. K., et al.; Mikrochim. Acta 191, 46 (2023), Abstract
Intranasal Administration of Forskolin and Noopept Reverses Parkinsonian Pathology in PINK1 Knockout Rats: R.K. Dagda, et al.; Int. J. Mol. Sci. 24, 690 (2023), Abstract
Intracellular sites of AQP2 S256 phosphorylation identified using inhibitors of the AQP2 recycling itinerary: P.W. Cheung, et al.; Am. J. Physiol. Renal Physiol. 324, F152 (2023), Abstract
Neuroinflammation-mediated mitochondrial dysregulation involved in postoperative cognitive dysfunction: Y. Yang, et al.; Free Radic. Biol. Med. 178, 134 (2022), Abstract
A grooved porous hydroxyapatite scaffold induces osteogenic differentiation via regulation of PKA activity by upregulating miR-129-5p expression: Deng, L., Li, X., et al.; J. Periodontal Res. 57, 1238 (2022), Abstract
Selective Targeting of Protein Kinase C (PKC)-θ Nuclear Translocation Reduces Mesenchymal Gene Signatures and Reinvigorates Dysfunctional CD8 + T Cells in Immunotherapy-Resistant and Metastatic Cancers: J. Dunn, et al.; Cancers 14, 1596 (2022), Abstract
Linagliptin prevents left ventricular stiffening by reducing titin cleavage and hypophosphorylation: Cuijpers, I., Papageorgiou, A. P., et al.; J. Cell. Mol. Med. 25, 729 (2021), Abstract
Forskolin regulates retinal endothelial cell permeability through TLR4 actions in vitro: L. Liu, et al.; Mol. Cell. Biochem. 476, 4487 (2021), Abstract

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PKA kinase activity kit

ADI-EKS-390A

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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SDS

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