MULTIVIEW® PLUS Antigen Retrieval Reagent (10X) (For Protease-Free Workflow)

• Protease-free — no Proteinase K required; protein epitopes preserved for IHC
• Enables ISH/IHC multiplexing: visualize protein and nucleic acid targets simultaneously on the same FFPE section
• Optimal for workflows requiring both RNA/DNA detection (ISH) and protein detection (IHC) without protease steps between staining layers
• High-pH heat-induced retrieval unmasks epitopes and hybridization targets in a single step
• Concentrated 10X format; dilute 1:10 for economical, consistent use
• Compatible with AMPIVIEW® ISH (LoopRNA ISH™) and Enzo POLYVIEW® IHC detection workflows

MULTIVIEW® Antigen Retrieval Reagent (10X) is a high-pH, heat-based epitope and nucleic acid target retrieval solution formulated for protease-free ISH and IHC workflows on formalin-fixed paraffin-embedded (FFPE) tissue sections. Unlike conventional ISH protocols that require Proteinase K (protease) digestion to unmask nucleic acid targets, MULTIVIEW® achieves effective target retrieval by preserving protein epitopes for simultaneous IHC detection. This makes it the ideal pre-treatment reagent for ISH/IHC multiplex assays, enabling visualization of both protein (antibody-based IHC) and nucleic acid (RNA or DNA ISH) targets in the same FFPE tissue section without protease treatment between staining steps. Supplied as a 10X concentrate; dilute 1:10 with deionized water before use. Compatible with AMPIVIEW® RNA ISH (LoopRNA ISH™ technology) and Enzo IHC detection workflows.

Formalin fixation forms protein–protein and protein–nucleic acid cross-links that mask both antibody epitopes and hybridization targets. Traditional ISH protocols use Proteinase K digestion to restore accessibility of nucleic acid targets; however, protease treatment degrades protein epitopes, making subsequent IHC on the same tissue section unreliable or impossible. Heat-induced antigen/target retrieval using a high-pH buffered solution reverses cross-linking through a protease-independent mechanism, unmasking both epitopes and hybridization targets simultaneously. This protease-free approach preserves protein antigenicity, enabling true ISH/IHC multiplexing — the simultaneous or sequential detection of RNA/DNA targets by ISH and protein markers by IHC on the same tissue section without the trade-off imposed by protease digestion.