Home Shop Lipid A from E. coli, Serotype R515 (Re) (TLRGRADE®) (Ready-to-Use)

Lipid A from E. coli, Serotype R515 (Re) (TLRGRADE®) (Ready-to-Use)

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TLR4 activator

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Activity	Activates Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice.
Couple Target	TLR, TLR4
Couple Type	Activator, Ligand
Formulation	Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
Purity	≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
Source	Isolated from E. coli R515 (Re mutant). Prepared by mild acid hydrolysis from purified Escherichia coli R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water.

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Keep sterile.
Long Term Storage	+4°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Quantum Molecular Resonance Inhibits NLRP3 Inflammasome/Nitrosative Stress and Promotes M1 to M2 Macrophage Polarization: Potential Therapeutic Effect in Osteoarthritis Model In Vitro: T. Paolucci, et al.; Antioxidants 12, 1358 (2023), Abstract
Mutant p53 suppresses innate immune signaling to promote tumorigenesis: Ghosh, M., Saha, S., et al.; Cancer Cell 39, 494 (2021), Abstract
Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death: J. Shi, et al.; Nature 526, 660 (2015), Application(s): Cell culture, Abstract
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract
R-form LPS, the master key to the activation of TLR4/MD-2-positive cells: M. Huber, et al.; methods in carbohy 36, 701 (2006), Abstract
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry 9, 11 (1993), Abstract
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975), Abstract
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969), Abstract