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This antibody is specifically designed to recognize and bind to human serum albumin, making it valuable in research and clinical settings for studying liver function, drug binding, and disease conditions where albumin levels are altered.
Human serum albumin is the most abundant protein in human blood plasma, playing a crucial role in maintaining osmotic pressure, transporting hormones, fatty acids, and other compounds.
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Flow cytometric analysis of Human HSA expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Human HSA in HepG2 cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSA monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
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RUO – Research Use Only |
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