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HIGHDEF^® red IHC chromogen (AP, plus)

ADI-950-141

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High definition IHC chromogen producing brilliant red color for AP-activated development in automated and manual systems.

HIGHDEF^® red IHC chromogen (AP, plus) is intended to be used in conjuction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems that run on automated staining instruments as well as manual use.

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HIGHDEF® red IHC chromogen (AP, plus) Immunohistochemistry — Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF^® IHC chromogen substrate (DAB, HC) (Prod. no. ADI-950-211, brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF^® blue IHC chromogen (AP) (Prod. no. ADI-950-150, blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF^® red IHC chromogen (AP, plus) (Prod. no. ADI-950-141, red), high molecular weight cytokeratin labeled with HIGHDEF^® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, yellow), and methyl green nuclear counterstain.

Product Details

Application	IHC, ISH (in situ hybridization)
Contents	HIGHDEF^® red IHC chromogen (AP, plus) substrate buffer and chromogen, Empty mixing bottle
Technical Info / Product Notes	Traditional formulations for red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic for use in automated slide strainers. The HIGHDEF^® red IHC chromogen (AP, plus) has been formulated for on-slide and on-board mixing for any autostainer. Both components are stable and can be loaded at the beginning of the staining run, for walk away convenience. It produces a red color, ranging from pink to brilliant dark red and is insoluble in organic solvents; therefore sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use.

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Spatiotemporal expression of fibroblast growth factor 4 and 10 during the morphogenesis of deciduous molars in miniature pigs: Y. Wang, et al.; Arch. Oral Biol. 155, 105795 (2023), Abstract
Inhibition of mitochondria ATP synthase suppresses prostate cancer growth through reduced insulin‐like growth factor‐1 secretion by prostate stromal cells: T. Ohishi, et al.; Int. J. Cancer 146, 3474 (2020), Abstract
CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy: W. Wang, et al.; Nature 569, 270 (2019), Abstract
Expression of programmed cell death ligand 1 is associated with poor overall survival in patients with diffuse large B-cell lymphoma: J. Kiyasu, et al.; Blood 126, 2193 (2015), Application(s): IHC using FFPE tissue, Abstract — Full Text

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HIGHDEF^® red IHC chromogen (AP, plus)

ADI-950-141

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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HIGHDEF® red IHC chromogen (AP, plus)

ADI-950-141

Product Details

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