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G418 . sulfate

ALX-380-013

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Antibiotic

Used to select neoR-positive cell lines and plasmids
Highly cited

G418 is a gentamycin-class aminoglycoside antibiotic with cytotoxic properties in both prokaryotic and eukaryotic cells. In a cell-free assay, G418 inhibits 80S ribosome and protein elongation during translation. In cells, G418 induces apoptosis and is widely employed in the selection of eukaryotic expression vectors containing the neomycin resistance gene neoR, in combination with either APH(2”) or APH(3′).

This product is the disulfide salt form of G418 CAS# 49863-47-0.

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Product Details

Activity	Potency: ≥700 µg/mg
Alternative Name	Geneticin
Appearance	White to off-white powder.
CAS	108321-42-2
Formula	C₂₀H₄₀N₄O₁₀ . 2H₂SO₄
Identity	Identity determined by HPLC and IR.
MW	496.6 . 196.1
RTECS	CB9378500
Solubility	Soluble in water.
Source	Isolated from Micromonospora rhodorangea.
Technical Info / Product Notes	The effective killing concentration of this antibiotic will vary as to the cell type, media, growth conditions, and the cells metabolic rate and position in the cell cycle. Effective concentrations have been reported from 100µg/ml up to 5mg/ml or greater. When using G418 in a new cell system, a full dose curve is suggested and with each new lot of G418, several points on that curve should be retested, as the potency determined in a standard B. subtilis assay may not exactly correlate to your system.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Solutions are stable for 3 to 6 months when stored at -20°C.
Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

A GluN2B disease-associated variant promotes degradation of NMDA receptors via autophagy: Benske, T. M., Williams, M. P., et al.; bioRxiv , (2025)
KIF2C-induced nuclear condensation concentrates PLK1 and phosphorylated BRCA2 at the kinetochore microtubules in mitosis: Skobelkina, A., Julien, M., et al.; bioRxiv , (2024)
Nanoparticles Based on Natural Lipids Reveal Extent of Impacts of Designed Physical Characteristics on Biological Functions: N. Kimura, et al.; ACS Nano 18, 1432 (2024), Abstract
Comparison of mutations in human parainfluenza viruses during passage in primary human bronchial/tracheal epithelial air-liquid interface cultures and cell lines: Sugimoto, S., Kawase, M., et al.; Microbiol. Spectr. 12, e0116424 (2024), Abstract
Preclinical studies of the triazolo[1,5-a]pyrimidine derivative WS-716 as a highly potent, specific and orally active P-glycoprotein (P-gp) inhibitor: S.Q. Wang, et al.; Acta Pharm. Sin. B 12, 3263 (2022), Abstract
Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABAA receptors: M. Wang, et al.; Cell Biosci. 12, 48 (2022), Abstract — Full Text
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors: A.L. Whittsette, et al.; iScience 25, 104754 (2022), Abstract — Full Text
Inhibition of gastric H+,K+-ATPase by new dihydropyrazole derivative KYY-008: T. Fujii, et al.; Biochem. Biophys. Res. Commun. 567, 177 (2021), Abstract
Overexpression of ABCC1 Confers Drug Resistance to Betulin: X. Chen, et al.; Front. Oncol. 11, 640656 (2021), Application(s): Cell treatment (carcinoma cell lines: HEK293), Abstract — Full Text
Pharmacokinetic engineering of OX40-blocking anticalin proteins using monomeric plasma half-life extension domains: M. Siegemund, et al.; Front. Pharmacol. 12, 759337 (2021), Abstract — Full Text