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Estrogen receptor α monoclonal antibody (33)

ALX-803-004

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Steroid receptors are a large superfamily of ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Estrogen receptor (ER) has been classified into two distinct isoforms, α and β. ERα is known to interact in a ligand dependent and independent manner with several known coactivators including Receptor-Interacting Protein 140 (RIP140/ERAP140), ERAP160, and Steroid Receptor Coactivator-1 (SRC-1) to enhance transcriptional activity of target genes.

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This antibody is covered by our Worry-Free Guarantee.

Prolactin receptor monoclonal antibody (U5) Immunohistochemistry — **Figure:** Western blot of recombinant ERa using MAb to Estrogen Receptor α (33) (Prod. No. ALX-803-004).”””,Estrogen receptor α monoclonal antibody (33) Western blot,Estrogen receptor α monoclonal antibody (33) Western blot,yes
ALX-803-005,803-005_color.gif,http://static.enzolifesciences.com/fileadmin/files/image/803-005_color.gif,**Figure:** Immunohistochemical staining of PRL receptor in rat Islets of Langerhans using MAb to Prolactin Receptor (U5) (Prod. No. ALX-803-005).”””””

Product Details

Alternative Name	ERα
Application	Flow Cytometry, ICC, IF, IHC (FS), IHC (PS), IP, WB
Application Notes	Immunohistochemistry: Staining of ERα in mouse Leydig cells results in both nuclear and cytoplasmic staining. Detects a band of ~67kDa by Western blot. Does not inhibit binding of ER to nuclei and DNA.
Clone	33
Crossreactivity	Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ERβ.
Formulation	Liquid. In Dulbecco’s PBS, containing 0.05% sodium azide, 1mg/mL BSA and 30% glycerol.
Host	Mouse
Immunogen	Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ERα (estrogen receptor α). This sequence is completely conserved in many species including mouse, rat, pig, bovine, sheep, chicken and frog.
Isotype	IgG1
Recommendation Dilutions/Conditions	Immunofluorescence (10µg/mL)Immunohistochemistry (1:20-1:100)Western Blot (4 µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Bovine, Human, Monkey, Mouse, Rat
Specificity	Recognizes both the steroid occupied and unoccupied forms of ERα.
UniProt ID	P03372
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Immunohistochemical localization of oestrogen receptor alpha in the various cell categories of chick embryo ovary: A. Civinini, et al.; Anat. Histol. Embryol. 39, 546 (2010), Application(s): Immunohistochemistry using chick embryo ovary, Abstract
Measurement of estrogen receptors in intact cells by flow cytometry: S. Cao, et al.; Cytometry 41, 109 (2000), Abstract
Histochemical and electrophysiological evidence for estrogen receptors on cultured astrocytes: colocalization with cholinergic receptors: E. Hosli, et al.; Int. J. Dev. Neurosci. 18, 101 (2000), Abstract
Cellular localization of estrogen receptors on neurones in various regions of cultured rat CNS: coexistence with cholinergic and galanin receptors: E. Hosli & L. Hosli; Int. J. Dev. Neurosci. 17, 317 (1999), Abstract
Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor: A. Traish, et al.; Steroids 55, 196 (1990), Abstract

Last modified: May 29, 2024

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Estrogen receptor α monoclonal antibody (33)

ALX-803-004

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