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Epithelial Cell Adhesion Molecule (EpCAM) is a cell surface protein involved in cell adhesion, proliferation, and differentiation. It is overexpressed in many epithelial cancers, making it a significant marker for tumor detection, cancer stem cells, and targeted therapies in oncology.
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Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.
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RUO – Research Use Only |
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