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Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification.
- Highly sensitive ELISA with an optional acetylation protocol increasing sensitivity >10-fold
- High troughput format with results in <3 hours for up to 39 samples in duplicate
- Widely cited in peer-reviewed publications
- Easy-to-use pre-coated plate and liquid color-coded reagents minimizes errors
- Simple, efficient and well-established sample handling protocols
- Highly reliable with consistent lot-to-lot performance
- Save with bulk kit package!
This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.
Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
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Typical standard curve used to calculate cAMP concentrations for Non-acetylated assay format.
Typical standard curve used to calculate cAMP concentrations for Acetylated assay format.
Product Details
| Alternative Name |
cyclic AMP |
|---|---|
| Application |
Colorimetric detection, ELISA |
| Application Notes |
For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include tissue culture. |
| Assay Time |
3 hours |
| Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
| Contents |
GxR Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine |
| Crossreactivity |
cyclic AMP (100%)AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%) |
| Sensitivity |
Non-acetylated: 1.18 pmol/ml (range 0.78 – 200 pmol/ml)Acetylated: 0.006 pmol/ml (range 0.078 – 20 pmol/ml) |
| Species Reactivity |
Species independent |
| Wavelength |
405 nm |
Handling & Storage
| Use/Stability |
Store all components at +4°C, except standard and conjugate at -20°C. |
|---|---|
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Begomovirus capsid proteins interact with cyclic adenosine monophosphate (cAMP)-specific phosphodiesterase of its whitefly vector and modulate virus retention within its vector: Ghosh, S., Mondal, B., et al.; J. Virol. 99, e0217224 (2025), Abstract
- Cyclic AMP Rescue of Motility in Sperm Devoid of Soluble Adenylyl Cyclase: Ayoub, S., Rivera Sanchez, N. D. R., et al.; Int. J. Mol. Sci. 26, 1489 (2025), Abstract
- Two unreported lecanoric acid derivatives isolated from Chaetomium globosum KM651986 and their anti-diabetic effect: R.A. Hussein, et al.; Sci. Rep. 15, 23554 (2025), Abstract — Full Text
- Orphan receptor GPR153 facilitates vascular damage responses by modulating cAMP levels, YAP/TAZ signaling, and NF-κB activation: Shao, J., Kwon, J., et al.; Nat. Commun. 16, 6232 (2025), Reactant(s): Human, Abstract
- The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice: de Oliveira do Rêgo, A. G., Maccari, S., et al.; Life Sci. Alliance 8, e202403094 (2025), Reactant(s): Mouse, Abstract
- Loss-of-function of GNAL dystonia gene impairs striatal dopamine receptors-mediated adenylyl cyclase/ cyclic AMP signaling pathway: I.E. Atiallah, et al.; Neurobiol. Dis. 191, 106403 (2024), Abstract
- Protein SUMOylation promotes cAMP-independent EPAC1 activation: W. Yang, et al.; bioRxiv , (2024), Abstract
- The phosphodiesterase 2A controls lymphatic junctional maturation via cGMP-dependent notch signaling: C. Carlantoni, et al.; Cell 59, 308 (2024), Abstract
- Chronic Stress-Induced Elevation of Melanin-Concentrating Hormone in the Locus Coeruleus Inhibits Norepinephrine Production and Associated With Depression-Like Behaviors in Rats: N. Kurban, et al.; Int. J. Neuropsychopharmacol. 27, pyad069 (2024), Abstract
- Distinct binding conformations of epinephrine with α- and β-adrenergic receptors: J.S. Lou, et al.; Exp. Mol. Med. 56, 1952 (2024), Abstract
- Dual role of valosin-containing protein (VCP/p97) in mouse sperm during capacitation: Jabloñski, M., La Spina, F. A., et al.; Reproduction 168, e240069 (2024), Abstract
- Ablation of GPR56 Causes β-Cell Dysfunction by ATP Loss through Mistargeting of Mitochondrial VDAC1 to the Plasma Membrane: I.M. Al-Amily, et al.; Biomolecules 13, 557 (2023), Abstract
- Ampicillin-controlled glucose metabolism manipulates the transition from tolerance to resistance in bacteria: M. Jiang, et al.; Sci. Adv. 9, eade8582 (2023), Abstract
- Cardiac contraction and relaxation are regulated by distinct subcellular cAMP pools: T.Y. Lin, et al.; Nat. Chem. Biol. , (2023), Abstract
- The Arabidopsis Deubiquitylase OTU5 Suppresses Flowering by Histone Modification-Mediated Activation of the Major Flowering Repressors FLC, MAF4, and MAF5: Y. Sato, et al.; Am. J. Respir. Cell Mol. Biol. 69, 281 (2023), Abstract
- The combined uterorelaxant effect of sildenafil and terbutalin in the rat: The potential benefit of co-administration of low doses: T. Barna, et al.; Heliyon 9, e22489 (2023), Abstract
- Transcription Factors Tec1 and Tec2 Play Key Roles in the Hyphal Growth and Virulence of Mucor lusitanicus Through Increased Mitochondrial Oxidative Metabolism: V.A. Castañeda, et al.; J. Microbiol. 61, 1043 (2023), Abstract
- Anti-Toxin Responses to Natural Enterotoxigenic Escherichia coli (ETEC) Infection in Adults and Children in Bangladesh: P. Girardi, et al.; Microorganisms 11, 2524 (2023), Abstract
- A cyclic adenosine monophosphate (cAMP)-specific phosphodiesterase interacts with begomovirus capsid proteins and modulates virus retention within its vector: Ghosh, S., Mondal, B., et al.; bioRxiv , (2023)
- Persistent Acidic Environment Induces Impaired Phagocytosis via ERK in Microglia: K.B. Jang, et al.; Neurochem. Res. , (2022), Abstract
- Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons: N.J. Yang, et al.; Nat. Neurosci. 25, 168 (2022), Abstract
- Glucagon signaling via supraphysiologic GCGR can reduce cell viability without stimulating gluconeogenic gene expression in liver cancer cells: J. Godfrey, et al.; Cancer Metab. 10, 4 (2022), Abstract
- Differential activation mechanisms of lipid GPCRs by lysophosphatidic acid and sphingosine 1-phosphate: S. Liu, et al.; Nat. Commun. 13, 731 (2022), Abstract
- REEP6 knockout leads to defective β-adrenergic signaling in adipocytes and promotes obesity-related metabolic dysfunction: Y. Son, et al.; Metabolism 130, 155159 (2022), Abstract
- A Series of Substituted Bis-Aminotriazines Are Activators of the Natriuretic Peptide Receptor C: R.J. Smith, et al.; J. Med. Chem. 65, 5495 (2022), Abstract
- EBI2 is a negative modulator of brown adipose tissue energy expenditure in mice and human brown adipocytes: F. Copperi, et al.; Commun. Biol. 5, 280 (2022), Abstract
- Adipocyte HIF2α functions as a thermostat via PKA Cα regulation in beige adipocytes: J.S. Han, et al.; Nat. Commun. 13, 3268 (2022), Abstract
- Regulator of G Protein Signaling Contributes to the Development and Aflatoxin Biosynthesis in Aspergillus flavus through the Regulation of Gα Activity: R. Xie, et al.; Appl. Environ. Microbiol. 88, e0024422 (2022), Abstract
- Harpagophytum procumbens Root Extract Mediates Anti-Inflammatory Effects in Osteoarthritis Synoviocytes through CB2 Activation: A. Mariano, et al.; Pharmaceuticals 15, 457 (2022), Abstract
- Structures of β1-adrenergic receptor in complex with Gs and ligands of different efficacies: M. Su, et al.; Nat. Commun. 13, 4095 (2022), Abstract
- P2RY14 cAMP signaling regulates Schwann cell precursor self-renewal, proliferation, and nerve tumor initiation in a mouse model of neurofibromatosis: J.P. Cram, et al.; Elife 11, e73511 (2022), Abstract
- Evaluation of Multivalent Enterotoxigenic Escherichia coli Vaccine Candidate MecVax Antigen Dose-Dependent Effect in a Murine Model: H. Seo, et al.; Appl. Environ. Microbiol. 88, e0095922 (2022), Abstract
- TSH-receptor autoantibodies in patients with chronic thyroiditis and hypothyroidism: M. Giannone, et al.; Clin. Chem. Lab. Med. 60, 1020 (2022), Abstract
- Anti-obesity effects of the dual-active adenosine A2A/A3 receptor-ligand LJ-4378: K. Kim, et al.; Int. J. Obes. 46, 2128 (2022), Abstract
- G protein-biased GPR3 signaling ameliorates amyloid pathology in a preclinical Alzheimer’s disease mouse model: Y. Huang, et al.; PNAS 119, e2204828119 (2022), Abstract
- Inhibition of PDE4 by apremilast attenuates skin fibrosis through directly suppressing activation of M1 and T cells: Q. Lu, et al.; Acta Pharmacol. Sinica 43, 376 (2022), Abstract
- A polyvalent multiepitope protein cross-protects against Vibrio cholerae infection in rabbit colonization and passive protection models: I. Upadhyay, et al.; PNAS 119, e2202938119 (2022), Abstract
- A positive feedback loop mediates crosstalk between calcium, cyclic nucleotide and lipid signalling in calcium-induced Toxoplasma gondii egress: S.D. Nofal, et al.; PLoS Pathog. 18, e1010901 (2022), Abstract
- The endocannabinoid anandamide is an airway relaxant in health and disease: A. Simon, et al.; Nat. Commun. 13, 6941 (2022), Abstract
- A three-dimensional culture system for generating cardiac spheroids composed of cardiomyocytes, endothelial cells, smooth-muscle cells, and cardiac fibroblasts derived from human induced-pluripotent stem cells: Kahn-Krell, A., Pretorius, D., et al.; Front. Bioeng. Biotechnol. 10, 908848 (2022), Abstract
- N-Formyl-L-aspartate mediates chemotaxis in sperm via the beta-2-adrenergic receptor: Panchal, D., Bhagwat, S., et al.; Front Cell Dev Biol 10, 959094 (2022), Abstract
- Resveratrol Treatment in Human Parkin-Mutant Fibroblasts Modulates cAMP and Calcium Homeostasis Regulating the Expression of Mitochondria-Associated Membranes Resident Proteins: A. Signorile, et al.; Biomolecules 11, 1511 (2021), Abstract
- β3 adrenergic receptor as potential therapeutic target in ADPKD: G. Schena, et al.; Physiol. Rep. 9, e15058 (2021), Abstract
- Glucagon transiently stimulates mTORC1 by activation of an EPAC/Rap1 signaling axis: S. Sunilkumar, et al.; Cell. Signal. 84, 110010 (2021), Abstract
- The level of secondary messengers and the redox state of NAD+/NADH are associated with sperm quality in infertility: S.N. Galimov, et al.; J. Reprod. Immunol. 148, 103383 (2021), Abstract
- Exhaustive endurance exercise activates brain glycogen breakdown and lactate production more than insulin-induced hypoglycemia: T. Matsui, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 320, 500 (2021), Abstract
- Rescue of cell surface expression and signalling of mutant follicle-stimulating hormone receptors: S. Hanyroup, et al.; Endocrinology 162, bqab134 (2021), Abstract
- Exendin-4 stimulates autophagy in pancreatic β-cells via the RAPGEF/EPAC-Ca2+-PPP3/calcineurin-TFEB axis: F.P. Zummo, et al.; Autophagy , (2021), Abstract
- Pre-clinical evaluation of dual targeting of the GPCRs CaSR and V2R as therapeutic strategy for autosomal dominant polycystic kidney disease: A. Di Mise, et al.; FASEB J. 35, 21874 (2021), Abstract
- Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity: M. Laudette, et al.; Cell Death Dis. 12, 4149 (2021), Abstract
- Functional evidence for biased inhibition of G protein signaling by YM-254890 in human coronary artery endothelial cells: Q. Peng, et al.; Eur. J. Pharmacol. 891, 173706 (2021), Application(s): Intra-cellular cAMP level with human coronary artery endothelial cells (HCAEC), Abstract
- Characterization of ShigETEC, a Novel Live Attenuated Combined Vaccine against Shigellae and ETEC: S. Harutyunyan, et al.; Vaccines (Basel) 8, 689 (2020), Abstract — Full Text
- Interleukin-1 receptor-induced PGE2 production controls acetylcholine-mediated cardiac dysfunction and mortality during scorpion envenomation: M.B. Reis, et al.; Nat. Commun. 11, 5433 (2020), Abstract — Full Text
- GPR56/ADGRG1 is a platelet collagen-responsive GPCR and hemostatic sensor of shear force: J. Yeung, et al.; PNAS 117, 28275 (2020), Abstract — Full Text
- Characterization of five novel vasopressin V2 receptor mutants causing nephrogenic diabetes insipidus reveals a role of tolvaptan for M272R-V2R mutation: F. Prosperi, et al.; Sci. Rep. 10, 16383 (2020), Abstract — Full Text
- Organogold drug Auranofin exhibits anti-melanogenic activity in B16F10 and MNT-1 melanoma cells: S. Goenka & S.R. Simon; Arch. Dermatol. Res. 312, 213 (2020), Application(s): cAMP quantification in B16-F10 (mouse melanoma) cell lysate., Abstract
- Vasoactive intestinal peptide axis is dysfunctional in patients with Graves’ disease: M. Carrion, et al.; Sci. Rep. 10, 13018 (2020), Abstract — Full Text
- Pannexin 1 inhibition delays maturation and improves development of Bos taurus oocytes: Z.T. Dye, et al.; J. Ovarian Res. 13, 98 (2020), Abstract — Full Text
- Inhibitory effect of UDP-glucose on cAMP generation and insulin secretion: F. Parandeh, et al.; J. Biol. Chem. 295, 15252 (2020), Abstract — Full Text
- Crth2 receptor signaling down-regulates lipopolysaccharide-induced NF-κB activation in murine macrophages via changes in intracellular calcium: B.T. Diwakar, et al.; FASEB J. 33, 12838 (2019), Abstract — Full Text
- Shear stress-induced endothelial adrenomedullin signaling regulates vascular tone and blood pressure: A. Iring, et al.; J. Clin. Invest. 130, 2775 (2019), Abstract — Full Text
- Regulation of AMPK activity by type 10 adenylyl cyclase: contribution to the mitochondrial biology, cellular redox and energy homeostasis: V. Jayarajan, et al.; Cell. Mol. Life Sci. 76, 4945 (2019), Abstract
- Elevated glucose concentration in culture media decreases membrane trafficking of SGLT2 in LLC-PK1 cells via a cAMP/PKA-dependent pathway: S. Sunilkumar, et al.; Am. J. Physiol. Cell Physiol. 316, C913 (2019), Abstract
- Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patients: R. Villanueva-Romero, et al.; Sci. Rep. 9, 7383 (2019), Abstract — Full Text
- Comparison of Five TSH-Receptor Antibody Assays in Graves’ disease: results from an observational pilot study: T. Struja, et al.; BMC Endocr. Disord. 19, 38 (2019), Abstract — Full Text
- Leucine-glycine and carnosine dipeptides prevent diabetes induced by multiple low-doses of streptozotocin in an experimental model of adult mice: T. Vahdatpour, et al.; J. Diabetes Investig. 10, 1177 (2019), Application(s): cAMP levels in blood samples of Male BALB/c mice, Abstract — Full Text
- Salsalate, but not metformin or canagliflozin, slows kidney cyst growth in an adult-onset mouse model of polycystic kidney disease: W.N. Leonhard, et al.; EBioMedicine 47, 436 (2019), Abstract — Full Text
- Obesity dysregulates fasting-induced changes in glucagon secretion: J.H. Stern, et al.; J. Endocrinol. 243, 149 (2019), Abstract
- Deletion of Osteopontin Enhances β2-Adrenergic Receptor-Dependent Anti-Fibrotic Signaling in Cardiomyocytes: C.M. Pollard, et al.; Int. J. Mol. Sci. 20, 1396 (2019), Abstract — Full Text
- Biphasic Effect of Sildenafil on Energy Sensing isMediated by Phosphodiesterases 2 and 3 inAdipocytes and Hepatocytes: J. Banerjee, et al.; Int. J. Mol. Sci. 20, 2992 (2019), Abstract — Full Text
- Mechanism of anti-remodelling action of treprostinil in human pulmonary arterial smooth muscle cells: C. Lambers, et al.; PLoS One 13, e0205195 (2018), Abstract — Full Text
- Human P2Y11 Expression Level Affects Human P2X7 Receptor-Mediated Cell Death: K. Dreisig, et al.; Front. Immunol. 9, 1159 (2018), Abstract — Full Text
- Anti-inflammatory and utero-relaxant effect of α-bisabolol on the pregnant human uterus: V.M. Munoz-Perez; Korean J. Physiol. Pharmacol. 22, 391 (2018), `, Abstract — Full Text
- Physiological Concentration of Prostaglandin E2 Exerts Anti-inflammatory Effects by Inhibiting Microglial Production of Superoxide Through a Novel Pathway: S.H. Chen, et al.; Mol. Neurobiol. 55, 8001 (2018), Abstract
- Pancreatic-derived factor impaired glucagon-like Peptide-1 production from GLUTag enterendorine L-cell line and intestines: F. Lai, et al.; Mol. Cell. Endocrinol. 452, 110 (2017), Application(s): GLUTag L-cell lysates and primary ileum lysates, Abstract
- Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injury: N.M. Wilson, et al.; PLoS One 12, e0178013 (2017), Abstract — Full Text
- Specific Combination of Salvianolic Acids As Core Active Ingredients of Danhong Injection for Treatment of Arterial Thrombosis and Its Derived Dry Gangrene: T. Zhao, et al.; Front. Pharmacol. 8, 361 (2017), Abstract — Full Text
- The Dietary Furocoumarin Imperatorin Increases Plasma GLP-1 Levels in Type 1-Like Diabetic Rats: L.Y. Wang, et al.; Nutrients 9, 1192 (2017), Application(s): CHO K1 cells, Abstract — Full Text
- LH prevents cisplatin-induced apoptosis in oocytes and preserves female fertility in mouse: V. Rossi, et al.; Cell Death Differ. 24, 72 (2017), Application(s): Lysates of mouse ovarian cultures, Abstract — Full Text
- Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression: X. Li, et al.; Theranostics 7, 4671 (2017), Application(s): Medium supernatant of Human umbilical vein endothelial cells (HUVECs), Abstract — Full Text
- Involvement of second messengers in the signaling pathway of vitellogenesis-inhibiting hormone and their effects on vitellogenin mRNA expression in the whiteleg shrimp, Litopenaeus vannamei: S.H. Bae, et al.; Gen. Comp. Endocrinol. 246, 301 (2017), Application(s): cAMP levels in shrimp ovaries, Abstract
- Escherichia coli O157: H7 suppresses host autophagy and promotes epithelial adhesion via Tir-mediated and cAMP-independent activation of protein kinase A: Y. Xue, et al.; Cell Death Discov. 3, 17055 (2017), Abstract
- Investigation of triamterene as an inhibitor of the TGR5 receptor: identification in cells and animals: Y. Li, et al.; Drug Des. Devel. Ther. 11, 1127 (2017), Application(s): Measurement of intracellular cAMP levels in rat NCI-H716 cells., Abstract — Full Text
- Aldosterone does not alter endothelin B receptor signaling in the inner medullary collecting duct: N. Ramkumar, et al.; Physio Rep. 5, e13167 (2017), Application(s): Measurement of cAMP in mouse inner medullary collecting duct cell line., Abstract — Full Text
- Hepatic adaptations to maintain metabolic homeostasis in response to fasting and refeeding in mice: C.E. Geisler, et al.; Nutr. Metab. (Lond.) 13, 62 (2016), Abstract — Full Text
- Neurotrophic and neuroprotective effects of oxyntomodulin in neuronal cells and a rat model of stroke: Y. Li, et al.; Exp Neurol. 14, 104 (2016), Application(s): Measurement of intracellular cAMP, Supernatants, Abstract
- Protective Effect of a GLP-1 Analog on Ischemia-Reperfusion Induced Blood-Retinal Barrier Breakdown and Inflammation: A. Goncalves, et al.; Invest. Ophthalmol. Vis. Sci. 57, 2584 (2016), Application(s): Intracellular levels of cAMP were measured, Abstract — Full Text
- Role of protein kinase A and class II phosphatidylinositol 3-kinase C2β in the downregulation of KCa3.1 channel synthesis and membrane surface expression by lyso-globotriaosylceramide: J.Y. Choi & S. Park; Biochem. Biophys. Res. Commun. 470, 907 (2016), Application(s): Measured cAMP levels, Abstract
- A Putative Association of a Single Nucleotide Polymorphism in GPR126 with Aggressive Periodontitis in a Japanese Population: J. Kitagaki, et al.; PLoS One 11, e0160765 (2016), Application(s): Intracellular cAMP measurement, HEK293T and HPDL cells, Abstract — Full Text
- Acute upregulation of neuronal mitochondrial type-1 cannabinoid receptor and it’s role in metabolic defects and neuronal apoptosis after TBI: Z. Xu, et al.; Mol. Brain 9, 75 (2016), Application(s): Cyclic AMP assay, isolated mitochondria, Abstract — Full Text
- Cortisol-induced immune suppression by a blockade of lymphocyte egress in traumatic brain injury: T. Dong, et al.; J. Neuroinflammation 13, 197 (2016), Application(s): Measurement of intracellular cAMP, mouse T cells, Abstract — Full Text
- Vibratory stimulation enhances thyroid epithelial cell function: A.P. Wagner, et al.; Biochem. Biophys. Rep. 8, 376 (2016), Application(s): FRTL-5 cells, Abstract — Full Text
- The Angiotensin-(1-7)/Mas axis improves pancreatic β-cell function in vitro and in vivo: A. Sahr, et al.; Endocrinology 157, 4677 (2016), Application(s): Determination of cyclic AMP (cAMP) concentrations, mouse lysates, Abstract
- Cyclic AMP effectors regulate myometrial oxytocin receptor expression: A. Yulia, et al.; Endocrinology 157, 4411 (2016), Application(s): Measurement of intracellular cAMP in myometrial tissue, Abstract — Full Text
- Protein Kinase A subunit balance regulates lipid metabolism in Caenorhabditis elegans and mammalian adipocytes: J.H. Lee, et al.; J. Biol. Chem. 291, 20315 (2016), Application(s): cAMP concentration measurement in Cenorhabditis elegans and mammalian cell lysates, Abstract — Full Text
- Phosphodiesterase Inhibition as a Mechanism of Ion Channel Modulation by Celecoxib: C.J. Robakowski; , (2016), (Master’s thesis); Application(s): Quantitative determination of cyclic AMP in cells treated with hydrochloric acid, Full Text
- New insight into the role of metabolic reprogramming in melanoma cells harboring BRAF mutations: A. Ferretta, et al.; Biochem. Biophys. Acta 1863, 2710 (2016), Application(s): Cyclic adenosine monophosphate (cAMP) assay in cell lysates, Abstract
- LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton: A.M. Sanchez, et al.; Mol. Cell. Endocrinol. 437, 22 (2016), Application(s): Measurement of cAMP in T-47D breast cancer cells, Abstract
- Increased food intake with oxyntomodulin analogues: S.L. Price, et al.; Peptides 73, 95 (2015), Abstract — Full Text
- Vasoactive intestinal peptide is a local mediator in a gut-brain neural axis activating intestinal gluconeogenesis: F. De Vadder, et al.; Neurogastroenterol. Motil. 27, 443 (2015), Application(s): Rat intenstine lysates, Abstract
- The aglycone of ginsenoside Rg3 enables glucagon-like peptide-1 secretion in enteroendocrine cells and alleviates hyperglycemia in type 2 diabetic mice: K.S. Kim, et al.; Sci. Rep. 5, 18325 (2015), Application(s): Measurement of intracellular cAMP, Abstract — Full Text
- Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling: K. Xie, et al.; eLife 4, e10451 (2015), Application(s): cAMP measurement of cell supernatants, Abstract — Full Text
- Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells: Y.C. Yoon, et al.; Molecules 20, 22128 (2015), Application(s): cAMP measurement in 3T3-L1 cells, Abstract — Full Text
- Investigating The Glucagon Receptor and GLP-1 Receptor Activity of Oxyntomodulin-Like Analogues in Male Wistar Rats: S.L. Price, et al.; Curr. Ther. Res. 77, 111 (2015), Application(s): ELISA using cell lysates, Abstract — Full Text
- Temporal cAMP signaling selectivity by natural and synthetic MC4R agonists: B.M. Molden, et al.; Mol. Endocrinol. 29, 1619 (2015), Application(s): cAMP determination by immunoassay, Abstract — Full Text
- MEK Inhibitors Reverse cAMP-Mediated Anxiety in Zebrafish: P.R. Lundegaard, et al.; Chem. Biol. 15, 330 (2015), Application(s):ELISA determining cAMP concentration in zebrafish larvae, Abstract
- Impaired enzymatic defensive activity, mitochondrial dysfunction and proteasome activation are involved in RTT cells oxidative damage: C. Cervellati, et al.; Biochim. Biophys. Acta. 1852, 2066 (2015), Application(s): ELISA using human supernatant, Abstract
- Ubiquitin is a versatile scaffold protein for the generation of molecules with de novo binding and advantageous drug-like properties: F. Job, et al.; FEBS Open Bio 5, 579 (2015), Application(s): ELISA using cell lysates, Abstract — Full Text
- The neuroprotective effect of lithium in cannabinoid dependence is mediated through modulation of cyclic AMP, ERK1/2 and GSK-3β phosphorylation in cerebellar granular neurons of rat: H.R. Rahimi, et al.; Iran. J. Pharm. Res. 14, 1123 (2015), Application(s): ELISA using rat supernatant, Abstract — Full Text
- Disruption of NHE8 Expression Impairs Leydig cell function in the testes: H. Xu, et al.; Am. J. Physiol. Cell Physiol. 308, C330 (2015), Application(s): Measurement of cAMP concentration in cell lysates, Abstract — Full Text
- The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM: F. Zhang, et al.; EMBO Rep. 16, 520 (2015), Application(s): ELISA, Abstract — Full Text
- Delocalization of Endogenous A-kinase Antagonizes Rap1-Rho-α2C-Adrenoceptor Signaling in Human Microvascular Smooth Muscle Cells: H.K. Motawea, et al.; J. Cytol. Mol. Biol. 1, 9 (2014), Application(s): Measurement of intracellular cAMP in human microVSM transduced with Ht31-P or Ht31, Abstract — Full Text
- Prostaglandin E2 promotes features of replicative senescence in chronically activated human CD8+ T cells: J.P. Chou, et al.; PLoS One 9, e99432 (2014), Application(s): EIA using cell culture supernatants, Abstract — Full Text
- Low concentrations of metformin suppress glucose production in hepatocytes through AMP-activated protein kinase (AMPK): J. Cao, et al.; J. Biol. Chem. 289, 20435 (2014), Application(s): cAMP levels in Cellular lysates were determined, Abstract — Full Text
- Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo: N. Fakhrudin, et al.; Br. J. Pharmacol. 171, 1676 (2014), Application(s): EIA using HUVECtert lysates (acetylated samples), Abstract — Full Text
- High throughput synthetic lethality screen reveals a tumorigenic role of adenylate cyclase in fumarate hydratase-deficient cancer cells: M. Boettcher, et al.; BMC Genomics 15, 158 (2014), Application(s): EIA using human hereditary leiomyomatosis and renal cell carcinoma (HLRCC) lysates, Abstract — Full Text
- Dopamine-2 receptor activation suppresses PACAP expression in gonadotrophs: S.J. Winters, et al.; Endocrinology 155, 2647 (2014), Application(s): Cell lysates were assayed, non-acetylated, Abstract — Full Text
- DCC is expressed in a CD166-positive subpopulation of chondrocytes in human osteoarthritic cartilage and modulates CRE activity: A.K. Bosserhoff, et al.; Int. J. Clin. Exp. Pathol. 7, 1947 (2014), Application(s): EIA using chondrocyte homogenates, Abstract — Full Text
- Cyclin-dependent kinase 5 in the ventral tegmental area regulates depression-related behaviors: P. Zhong, et al.; J. Neurosci. 34, 6352 (2014), Application(s): EIA using mouse ventral tegmental area (VTA) homogenates, Abstract — Full Text
- Regulator of G-protein signaling 6 (RGS6) promotes anxiety and depression by attenuating serotonin-mediated activation of the 5-HT(1A) receptor-adenylyl cyclase axis: A. Stewart, et al.; FASEB J. 28, 1735 (2014), Application(s): cAMP Measurements were performed, Abstract — Full Text
- Resveratrol and curcumin enhance pancreatic β-cell function by inhibiting phosphodiesterase activity: M. Rouse, et al.; J. Endocrinol. 223, 107 (2014), Application(s): EIA using mouse β-Min6 lysates and primary human islet lysates, Abstract — Full Text
- Three intragenic suppressors of a GTPase-deficient allele of GNAS associated with McCune-Albright syndrome: K. Turcic, et al.; J. Mol. Endocrinol. 52, 321 (2014), Application(s): Assaying levels of cAMP in lysates, Abstract
- The metabolite 5′-methylthioadenosine signals through the adenosine receptor A2B in melanoma: K. Limm, et al.; Eur J. Cancer 50, 2714 (2014), Application(s): Quantification of intracellular cAMP, Abstract
- Adenosine 2A receptor promotes collagen production by human fibroblasts via pathways involving cyclic AMP and AKT but independent of Smad2/3: M. Perez-Aso, et al.; FASEB J. 28, 802 (2014), Application(s): Intracellular cAMP was measured, Abstract — Full Text
- Global analysis of the HrpL regulon in the plant pathogen Pseudomonas syringae pv. tomato DC3000 reveals new regulon members with diverse functions: H.N. Lam, et al.; PLoS One 9, e106115 (2014), Application(s): EIA using leaf extracts from N. benthamiana, Abstract — Full Text
- Lipid droplet protein LID-1 mediates ATGL-1-dependent lipolysis during fasting in Caenorhabditis elegans: J.H. Lee, et al.; Mol. Cell. Biol. 34, 4165 (2014), Application(s): cAMP concentrations were measured, Abstract — Full Text
- Rolofylline, an adenosine A1 receptor antagonist, inhibits osteoclast differentiation as an inverse agonist: W. He, et al.; Br. J. Pharmacol. 170, 1167 (2013), Application(s): EIA using bone marrow macrophage lysates (acetylated samples), Abstract — Full Text
- Resveratrol prevents β-cell dedifferentiation in nonhuman primates given a high-fat/high-sugar diet: J.L. Fiori, et al.; Diabetes 62, 3500 (2013), Application(s): EIA using CHO cell supernatant, Abstract — Full Text
- Molecular mechanisms underlying the rapid arrhythmogenic action of bisphenol A in female rat hearts: X. Gao, et al.; Endocrinology 154, 4607 (2013), Application(s): EIA using rat myocyte homogenates, Abstract — Full Text
- Does 4-tert-octylphenol affect estrogen signaling pathways in bank vole Leydig cells and tumor mouse Leydig cells in vitro?: M. Kotula-Balak, et al.; Reprod. Toxicol. 39, 6 (2013), Application(s): cAMP was measured and quantitated, Abstract
- Age-dependent alterations in cAMP signaling contribute to synaptic plasticity deficits following traumatic brain injury: D.J. Titus, et al.; Neuroscience 231, 182 (2013), Application(s): EIA using rat cortex, hippocampus and thalamus homogenates, Abstract — Full Text
- Liraglutide protects against amyloid-β protein-induced impairment of spatial learning and memory in rats: W.N. Han, et al.; Neurobiol Aging 34, 576 (2013), Application(s): EIA using rat brain homogenates, Abstract
- Calcium supplementation increases circulating cholesterol by reducing its catabolism via GPER and TRPC1-dependent pathway in estrogen deficient women: S. Li, et al.; Int. J. Cardiol. 168, 2548 (2013), Application(s): ELISA using rat liver tissue, Abstract
- Role of AKAP79/150 protein in b1-adrenergic receptor trafficking and signaling in mammalian cells: X. Li, et al.; J. Biol. Chem. 288, 33797 (2013), Application(s): ELISA using murine cardiomyocytes, Abstract
- Similarities and differences in interactions of thyroid stimulating and blocking autoantibodies with the TSH receptor: R. Núñez Miguel, et al.; J. Mol. Endocrinol. 49, 137 (2012), Application(s): ELISA – CHO cell lysate, Abstract — Full Text
- Gonadotropin-inhibitory hormone inhibits GnRH-induced gonadotropin subunit gene transcriptions by inhibiting AC/cAMP/PKA-dependent ERK pathway in LβT2 cells: Y.L. Son, et al.; Endocrinology 153, 2332 (2012), Application(s): Measurement of cAMP accumulation in cells, Abstract — Full Text
- Constitutive cholesterol-dependent endocytosis of melanocortin-4 receptor (MC4R) Is essential to maintain receptor responsiveness to α-melanocyte-stimulating hormone (α-MSH): F.K. McDaniel, et al.; J. Biol. Chem. 287, 21873 (2012), Application(s): ELISA using human neuronal cells, Abstract
- Regulation of pancreatic cancer by neuropsychological stress responses: a novel target for intervention: H.M. Schuller, et al.; Carcinogenesis 33, 191 (2012), Application(s): ELISA using human pancreatic cancer xenografts, Abstract
- Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets: H.P. de Koning, et al.; J. Infect. Dis. 206, 229 (2012), Application(s): EIA using Trypanosoma lysates, Abstract — Full Text
- Rescue of expression and signaling of human luteinizing hormone G protein-coupled receptor mutants with an allosterically binding small-molecule agonist: C.L. Newton, et al.; PNAS 108, 7172 (2011), Application(s): Measured level of cAMP in cell supernatants, Abstract — Full Text
- Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress: S.J. White, et al.; J. Cell. Physiol. 226, 2841 (2011), Application(s): ELISA using HUVEC, Abstract — Full Text
- Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase γ and phosphodiesterase 3B: A. Baragli, et al.; Biochim. Biophys. Acta 1811, 386 (2011), Application(s): EIA using rat adipocyte extracts, Abstract
- cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase: J. Chen, et al.; Reproduction 138, 759 (2009), Abstract
- PPARγ agonists inhibit vasopressin-mediated anion transport in the MDCK-C7 cell line: C. Nofziger, et al.; Am. J. Physiol. Renal Physiol. 297, F55 (2009), Application(s): ELISA using MDCK-C7 cell lysates, Abstract — Full Text
- Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells: P. Hardwidge, et al.; J. Bacteriol. 191, 178 (2009), Application(s): EIA using porcine cell lysates, Abstract
- Androgens transduce the G-alpha s-mediated activation of protein kinase A in prostate cells: Y. Daaka, et al.; Cancer Res. 68, 3225 (2008), Application(s): EIA using human cell lysates, Abstract
- Cyclic Adenosine Monophosphate Regulation of Ion Transport in Porcine Vocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008), Application(s): Measured cAMP concentrations in porcine samples, Abstract — Full Text
- Functional evidence for presence of lipid rafts in erythrocyte membranes: Gs-alpha in rafts is essential for signal transduction: Y. Takakuwa, et al.; Am. J. Hematol. 83, 371 (2008), Application(s): EIA using human cell lysates, Abstract
- beta-Arrestin-1 mediates glucagon-like peptide-1 signaling to insulin secretion in cultured pancreatic beta cells: J. Olefsky, et al.; PNAS 105, 6614 (2008), Application(s): EIA using rat cell lysates, Abstract
- FMRP acts as a key messenger for dopamine modulation in the forebrain: M. Zhao, et al.; Neuron 59, 634 (2008), Application(s): EIA using mouse cell lysates, Abstract
- Regulation of G proteins by human 5-HT1a receptor TM3/i2 and TM5/i3 loop peptides: H.V. Thiagaraj, et al.; Neurochem. Int. 50, 109 (2007), Application(s): EIA using human cell lysates, Abstract
- The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator: T. Petnicki-Ocwieja, et al.; J. Bacteriol. 187, 649 (2005), Application(s): EIA using plant cell lysates, Abstract
- Co-occurrence of Two Partially Inactivating Polymorphisms of MC3R Is Associated With Pediatric-Onset Obesity: N. Feng, et al.; Diabetes 54, 2663 (2005), Application(s): EIA using human cell lysates, Abstract
- High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al.; Am. J. Physiol. Heart Circ. Physiol. 284, H1124 (2003), Application(s): EIA using rat cell lysates, Abstract
- Lysine 270 in the Third Intracellular Domain of the Oxytocin Receptor is an Important Determinant for G{alpha}q Coupling Specificity: M. Yang, et al.; Mol. Endocrinol. 16, 814 (2002), Application(s): EIA using monkey cell lysates, Abstract
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