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DEEPSEE® NRG1 Break Apart FISH Probe

ENZ-GEN466

Datasheet
Manual
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Ready-to-use directly labeled FISH probe for NRG1 Break Apart detection

Directly labeled, ready-to-use probe
2-hour hybridization; same-day results
US manufactured with 24–48 hour delivery for uninterrupted lab workflow

The DEEPSEE® NRG1 Break Apart FISH Probe is a directly labeled, ready-to-use fluorescence in situ hybridization (FISH) probe for the detection of chromosomal copy number alterations and/or structural rearrangements at the NRG1 locus. The probe is supplied as a pre-diluted hybridization solution in formamide-based buffer and is compatible with automated hybridization instruments (HYBrite™ / ThermoBrite®). This break-apart probe flanks the NRG1 gene with Green / Orange labeled sequences. Structural rearrangements at this locus are indicated by separation of the two signal colors. SEEBRIGHT® Mounting Medium with DAPI is an antifade reagent that minimizes photobleaching. Use of this mounting medium significantly extends the lifetime of fluorescent signals. The inclusion of DAPI allows for staining of the nucleus.

Supplied as 20 tests (200 µL) of ready-to-use probe solution.

Shipping: Available products typically ship within 24/48h, via priority shipping.

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Product Details

Application	FISH
Assay Time	3 hr Total run time (2-hour hybridization); same-day results
Contents	1 × DEEPSEE® NRG1 Break Apart FISH Probe (ENZ-GEN466-0020), 20 tests (200 µL) ready-to-use probe solution in formamide-based buffer Dye Specifications: Probes labeled with Red-dUTP (Ex 580 nm/ Em 599 nm) Probes labeled with Green-dUTP (Ex 491 nm/ Em 516 nm) Probes labeled with Orange-dUTP (Ex 552 nm/ Em 576 nm) Probes labeled with Gold-dUTP (Ex 525 nm/ Em 551 nm) Probes labeled with Aqua-dUTP (Ex 434 nm/Em 480 nm)

Application

FISH

Assay Time

3 hr Total run time (2-hour hybridization); same-day results

Contents

1 × DEEPSEE® NRG1 Break Apart FISH Probe (ENZ-GEN466-0020), 20 tests (200 µL) ready-to-use probe solution in formamide-based buffer

Dye Specifications:
Probes labeled with Red-dUTP (Ex 580 nm/ Em 599 nm)
Probes labeled with Green-dUTP (Ex 491 nm/ Em 516 nm)
Probes labeled with Orange-dUTP (Ex 552 nm/ Em 576 nm)
Probes labeled with Gold-dUTP (Ex 525 nm/ Em 551 nm)
Probes labeled with Aqua-dUTP (Ex 434 nm/Em 480 nm)

Handling & Storage

Use/Stability	When used and stored as directed, reagents are stable for 1 year from date of shipment as per the date stated on the product label. Avoid freeze-thaw cycles.
Handling	Protect from light and humidity at all times. Store at −20°C. Avoid freeze-thaw cycles. Centrifuge briefly before use.
Short Term Storage	4 °C
Long Term Storage	-20°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Specific Protocol

Refer to the enclosed Instruction Manual for complete slide pretreatment, denaturation, hybridization, post-hybridization wash, and mounting procedures. Manual protocol: denaturation at 75°C, hybridization at 37°C for 14–18 hours. Automated co-denaturation protocol (ThermoBrite® / HYBrite™): denaturation at 76°C for 3 minutes, hybridization at 37°C for approximately 2 hours.

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SDS

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