DEEPSEE® ETV6 Break Apart FISH Probe

• Directly labeled, ready-to-use probe
• 2-hour hybridization; same-day results
• US manufactured with 24–48 hour delivery for uninterrupted lab workflow

The DEEPSEE® ETV6 Break Apart FISH Probe is a directly labeled, ready-to-use fluorescence in situ hybridization (FISH) probe for the detection of chromosomal copy number alterations and/or structural rearrangements at the ETV6 locus. The probe is supplied as a pre-diluted hybridization solution in formamide-based buffer and is compatible with automated hybridization instruments (HYBrite™ / ThermoBrite®). This break-apart probe flanks the ETV6 gene with Green / Orange labeled sequences. Structural rearrangements at this locus are indicated by separation of the two signal colors. SEEBRIGHT® Mounting Medium with DAPI is an antifade reagent that minimizes photobleaching. Use of this mounting medium significantly extends the lifetime of fluorescent signals. The inclusion of DAPI allows for staining of the nucleus.

Supplied as 20 tests (200 µL) of ready-to-use probe solution.

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescently labeled DNA probes to detect and localize specific DNA sequences on chromosomes. FISH is widely used in research and clinical laboratories for the detection of chromosomal copy number changes, gene amplifications, deletions, and structural rearrangements associated with cancer and genetic disorders. DEEPSEE® directly labeled FISH probes are supplied as pre-diluted hybridization solutions, enabling high-sensitivity detection without additional labeling steps. SEEBRIGHT® Mounting Medium with DAPI is recommended for use with this probe.