Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
This antibody is covered by our Worry-Free Guarantee.

Immunohistochemistry analysis of human skin tissue using Cytokeratin 1/5/10/14 (human), mAb (34βE12), (Prod. No. ENZ-30903).



Product Details
Alternative Name |
HMWCK, High molecular weight cytokeratin, Keratin-903 |
---|---|
Application |
IHC (PS), WB |
Clone |
34βE12 |
Formulation |
Liquid. Ready-to-use solution in PBS containing 0.1% sodium azide and 1% BSA. |
Host |
Mouse |
Immunogen |
Keratin solubilized from human stratum corneum. |
Isotype |
IgG1 |
Purity Detail |
Affinity purified. |
Recommendation Dilutions/Conditions |
Immunohistochemistry (paraffin sections; for optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37°C for 2 to 4 hours. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Source |
Purified from ascites. |
Species Reactivity |
Human |
Specificity |
34βE12 is specific for “high molecular weight” cytokeratins 1, 5, 10 and 14, corresponding to molecular weights 68, 58, 56.5 and 50 kDa, respectively, which are characteristically found in “complex” epithelium. The antibody reacts with all squamous and ductal epithelium as well as carcinomas such as squamous cell carcinoma and adenocarcinoma of ductal origin (e.g., breast, pancreas) but does not react with sarcoma, melanoma or lymphoma. This antibody reacts with benign small-acinar lesions of the prostate and has been shown to be useful as an adjunct in the identification of prostate carcinoma. |
UniProt ID |
P04264 (CK1), P13647 (CK5), P13645 (CK10), P02533 (CK14) |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Handling |
Avoid freeze/thaw cycles. |
---|---|
Long Term Storage |
+4°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
---|
- Allogeneic transplantation of epidermal cell sheets followed by endoscopic submucosal dissection to prevent severe esophageal stricture in a porcine model: S. Kobayashi, et al.; Regen. Ther. 21, 157 (2022), Abstract
- Identification of Small Molecule Enhancers of Immunotherapy for Melanoma: C. Dextras, et al.; Sci. Rep. 10, 5688 (2020), Application(s): IHC, Abstract — Full Text
- Prostate cancer cell telomere length variability and stromal cell telomere length as prognostic markers for metastasis and death: C.M. Heaphy, et al.; Cancer Discov. 3, 1130 (2013), Application(s): Immunohistochemistry multiplexed with fluorescent in-situ hybridization using formalin-fixed, paraffin-embedded human prostate carcinoma tissue samples, Abstract — Full Text
- Cytoplasmic PTEN protein loss distinguishes intraductal carcinoma of the prostate from high-grade prostatic intraepithelial neoplasia: T.L. Lotan, et al.; Mod. Pathol. 26, 587 (2013), Application(s): Multiplex immunohistochemistry using formalin-fixed, paraffin-embedded human prostate carcinoma tissue samples, Abstract — Full Text
- Immunohistochemical markers for the differential diagnosis of nephrogenic adenomas: W. Quinones, et al.; Ann. Diagn. Pathol. 17, 41 (2013), Application(s): Immunohistochemistry using formalin-fixed, paraffin-embedded human nephrogenic adenoma tissue samples, Abstract
- Primary lymphoepithelioma-like carcinoma of the ureter: D.S. Allende, et al.; Ann. Diagn. Pathol. 14, 209 (2010), Abstract
- Immunohistochemical Staining with DeltaNp63 Is Useful for Distinguishing the Squamous Cell Component of Adenosquamous Cell Carcinoma of the Lung: H. Uramoto, et al.; Anticancer Res. 30, 4717 (2010), Application(s): IHC using human lung cancer (ADSQ) samples, Abstract — Full Text
- Somatic but Not Germline Mutation of the APC Gene in a Case of Cribriform-Morular Variant of Papillary Thyroid Carcinoma: J. Cameselle-Teijeiro, et al.; Am. J. Clin. Pathol. 115, 486 (2001), Application(s): IHC using human thyroid carcinoma samples, Abstract — Full Text
- Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18: D. Bello, et al.; Carcinogenesis 18, 1215 (1997), Application(s): ICC using human prostate cell lines RWPE-1 and -2, Abstract — Full Text
- Metaplastic Shadow Cells in Rectal Adenocarcinoma: Report of a Case With Immunohistochemical Study: H. Nakayama, et al.; Jpn. J. Clin. Oncol. 27, 427 (1997), Application(s): IHC using human rectal adenocarcinoma, Abstract — Full Text
- Use of keratin 903 as an adjunct in the diagnosis of prostate carcinoma: L. Hedrick & J.I. Epstein; Am. J. Surg. Pathol. 13, 389 (1989), Abstract
- Basal cell hyperplasia, adenoid basal cell tumor, and adenoid cystic carcinoma of the prostate gland: an immunohistochemical study: D.J. Grignon, et al.; Hum. Pathol. 19, 1425 (1988), Abstract
- Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues: A.M. Gown & A.M. Vogel; Am. J. Pathol. 114, 309 (1984), Abstract — Full Text
- Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies: A.M. Gown & A.M. Vogel; J. Cell Biol. 95, 414 (1982), Abstract — Full Text
Related Products

Alternative Name | CK8 |
---|---|
Application | IHC (PS), WB |
Host | Mouse |
Isotype | IgM |
Species Reactivity | Human |

Alternative Name | HMWCK, High molecular weight cytokeratin, Keratin-903 |
---|---|
Application | IHC (PS), WB |
Host | Mouse |
Isotype | IgG1 |
Species Reactivity | Human |
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
Please enter the lot number as featured on the product label
SDS
Enzo Life Science provides GHS Compliant SDS
If your language is not available please fill out the SDS request form