Collagenase AF-1 GMP Grade from Clostridium histolyticum

Collagenase ≥ 150 U/Vial or ≥ 2000 U/Vial (PZ activity according to Wünsch, 25 °C)Collagenase ≥ 3000 U/mg lyophilisate (PZ activity according to Wünsch, 25 °C)Neutral Protease ≤ 0.050 U/mg lyophilisate (DMC assay)PZ Unit (acc. to Wünsch):1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.Neutral Protease:1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS.

9001-12-1

Endotoxin*:Sterility (Ph. Eur.)Absence of ClostridiaTAMC (Total Aerobic Microbial Count) ≤ 10/VialTYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

70 – 120 kDa (collagenases)

Manufactured free from animal components & according to international GMP guidelines.

From Clostridium histolyticum. Contains Collagenase class l and class ll.

NB-17457-01 corresponds to supplier’s Cat. No. N0003554

For the Original Manufacturer’s data sheet please click here.