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Immunofluorescence staining of CDK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CDK4 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
Immunochemical staining of human CDK4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
CDK4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg k562 Whole Cell Lysate Lane C:0.5 mg Hela Whole Cell Lysate, 2 µL anti-CDK4 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CDK4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Observed band size: 34 kDa
Anti-CDK4 rabbit polyclonal antibody at 1:500 dilution. Lane A: CDK4 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:33 kDa.
Anti-CDK4 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate: Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Observed band size:33 kDa.
| Regulatory Status |
RUO – Research Use Only |
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