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Cathepsin D & E substrate (fluorogenic)

BML-P145

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Synthetic substrate useful for inhibitor screening and kinetic analysis

Quenched fluorogenic substrate for cathepsin D (k_cat/K_m=1.09 x 10 M^-1s^-1) and cathepsin E (k_cat/K_m=1.56 x 10 M^-1s^-1). Mca fluorescence is quenched by the Dnp group until cleavage separates them. Activity of the two enzymes on this substrate can be distinguished by the use of Ascaris pepsin inhibitor, which inhibits cathepsin E, but not cathepsin D. This substrate is also cleaved by MMP-2, MMP-3, MMP-9, but not MMP-1 nor MMP-13. This substrate is useful for inhibitor screening and kinetic analysis. Ex.: 320-340 nm, Em.: 393-420 nm. Mca-Pro-Leu-OH (BML-P127) can be used to calibrate for Mca fluorescence.

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Product Details

Appearance	Yellow powder
Formulation	Trifluroacetate salt
MW	1756.0
Purity	≥95% (HPLC)
Quality Control	Determined by MS.
Sequence	Mca-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂ [Mca= (7-methoxycoumarin-4-yl)acetyl; Dnp=dinitrophenyl]
Solubility	Soluble in DMSO (1mM) or 50% acetonitrile (10mg/ml).
Source	Synthetic

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light. Keep cool and dry.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Late-onset Krabbe disease presenting as spastic paraplegia – implications of GCase and CTSB/D: Mächtel, R., Dobert, J. P., et al.; Ann. Clin. Transl. Neurol. 11, 1715 (2024), Abstract
Adaptive responses of neuronal cells to chronic endoplasmic reticulum (ER) stress: T.N.M. Pham, et al.; Redox Biol. 67, 102943 (2023), Abstract
USP17 is required for peripheral trafficking of lysosomes: J. Lin, et al.; EMBO Rep. , e51932 (2022), Abstract
The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells: M. Oroń, et al.; Cell Rep. 40, 111428 (2022), Abstract
Cathepsin D: Analysis of its potential role as an amyloid beta degrading protease: L. Gallwitz, et al.; Neurobiol. Dis. 175, 105919 (2022), Abstract
Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons: P.P.Y. Lie, et al.; Cell Rep. Med. 35, 109034 (2021), Abstract
Analysis of cathepsin B and cathepsin L treatment to clear toxic lysosomal protein aggregates in neuronal ceroid lipofuscinosis: A. Di Spiezio, et al.; Biochim. Biophys. Acta Mol. Basis Dis. 1867, 166205 (2021), Abstract
Trehalose Reduces the Secreted Beta-Amyloid Levels in Primary Neurons Independently of Autophagy Induction: I. Benito-Cuesta, et al.; Metabolites 11, 421 (2021), Abstract
Dictyostelium lacking the single atlastin homolog Sey1 shows aberrant ER architecture, proteolytic processes and expansion of the Legionella-containing vacuole: Hüsler, D., Steiner, B., et al.; Cell. Microbiol. 23, e13318 (2021), Abstract
Transgenic expression of GFP-LC3 perturbs autophagy in exocrine pancreas and acute pancreatitis responses in mice: Mareninova, O. A., Jia, W., et al.; Autophagy 16, 2084 (2020), Reactant(s): Mouse, Abstract

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Cathepsin D & E substrate (fluorogenic)

BML-P145

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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