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- Trusted – regularly cited in peer-reviewed publications
- Sensitive – optional acetylation protocol increases sensitivity >10-fold (0.027 pmol/ml)
- Rapid – results in just 3 hours from up to 39 samples in duplicate
- Easy-to-use – pre-coated plate and liquid color-coded reagents reduce errors
- Simple – efficient and well-established sample handling protocols
- Reliable – consistent lot-to-lot performance
This is a colorimetric competitive enzyme immunoassay kit for the quantitative determination of extracellular cAMP. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error.
Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
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Typical standard curve used to calculate cAMP concentrations for Acetylated assay format.
Typical standard curve used to calculate cAMP concentrations for Non-acetylated assay format.
| Regulatory Status |
RUO – Research Use Only |
|---|
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Direct cAMP ELISA kit
ADI-900-066A
Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification.
| Application | Colorimetric detection, ELISA |
|---|---|
| Assay Time | 3 hours |
| Sensitivity | Non-acetylated: 1.18 pmol/ml (range 0.78 – 200 pmol/ml)Acetylated: 0.006 pmol/ml (range 0.078 – 20 pmol/ml) |
| Species Reactivity | Species independent |
cAMP complete ELISA kit
ADI-900-163A
Most sensitive (0.006 pmol/ml and 0.027 pmol/ml) and complete colorimetric kit for intra- and extracellular cAMP quantification.
| Application | Colorimetric detection, ELISA |
|---|---|
| Assay Time | 3 hours |
| Sensitivity | Non-acetylated: 0.49 pmol/ml (assay buffer) / 1.18 pmol/ml (HCl) (range (non-acetylated) 0.78 – 200 pmol/ml)Acetlylated: 0.027 pmol/ml (assay buffer) / 0.006 pmol/ml (HCl) (range (acetylated) 0.078 – 20 pmol/ml) |
| Species Reactivity | Species independent |
Direct cAMP ELISA kit
ADI-901-066A
Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification.
| Application | Colorimetric detection, ELISA |
|---|---|
| Assay Time | 3 hours |
| Sensitivity | Non-acetylated: 1.18 pmol/ml (range 0.78 – 200 pmol/ml)Acetylated: 0.006 pmol/ml (range 0.078 – 20 pmol/ml) |
| Species Reactivity | Species independent |
cAMP ELISA kit
ADI-901-067A
Most sensitive (0.027 pmol/ml) colorimetric kit for extracellular cAMP quantification.
| Application | Colorimetric detection, ELISA |
|---|---|
| Assay Time | 3 hours |
| Sensitivity | Non-acetylated: 0.49 pmol/ml (range 0.78 – 200 pmol/ml)Acetylated: 0.027 pmol/ml (range 0.078 – 20 pmol/ml) |
| Species Reactivity | Species independent |