cAMP complete ELISA kit

• Versatile and convenient kit used to assay both intracellular and extracellular cAMP
• Highy sensitive measurement of cAMP with an optional acetylation protocol increasing sensitivity >10-fold (0.006 pmol/ml and 0.027 pmol/ml) for intra- and extracellular cAMP, respectively
• Simple, efficient and well-established sample handling protocols
• Widely cited in peer-reviewed publications
• Higher throughput format with results in 3 hours for up to 39 samples in duplicate
• Reliable and consistent lot-to-lot performance
• Save with bulk kit package!

This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.

Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.