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BMPO (high purity)

ALX-430-141

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Spin trapping reagent

Highly pure spin trapping reagent
Contains very low paramagnetic impurities and does not require further purification
Comes as a crystalline solid for a longer shelf-life

BMPO is a cyclic nitrone spin trap for the specific in vivo or in vitro detection of short-lived hydroxyl, superoxide, and thiyl radicals. BMPO forms distinguishable adducts that can be measured by EPR spectroscopy. Unlike with DMPO (Prod. No. ALX-430-090), the superoxide adduct does not decay into a hydroxyl adduct and has a much longer half-life in cells (t_1/2=23min).

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Product Details

Alternative Name	5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide
Appearance	White to pinkish crystalline solid.
CAS	387334-31-8
Formula	C₁₀H₁₇NO₃
Identity	Identity determined by MS and NMR.
MW	199.3
Purity	≥99% (HPLC)
Solubility	Soluble in DMSO (25mg/ml), 100% ethanol, 100% methanol, and water.
Source	Synthetic

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from moisture.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Dynamic Wood Smoke Aerosol Toxicity during Oxidative Atmospheric Aging: S. Wang, et al.; Environ. Sci. Technol. 3, 1246 (2023), Abstract
Hydrothermal synthesis of PVP-passivated clove bud-derived carbon dots for antioxidant, catalysis, and cellular imaging applications: A.K. Pandey, et al.; Colloids Surf. B Biointerfaces 220, 112926 (2022), Abstract
Effects of acidity on reactive oxygen species formation from secondary organic aerosols: J. Wei, et al.; ACS Environ. Au. 2, 336 (2022), Abstract
Activation of peroxymonosulfate by bicarbonate and acceleration of the reaction by freezing: Y.Y. Ah, et al.; Sci. Total Environ. 785, 147369 (2021), Abstract
Oxidants produced by methylglyoxal-modified collagen trigger ER stress and apoptosis in skin fibroblasts: K. Nowotny, et al.; Free Radic. Biol. Med. 120, 102 (2018), Abstract
Reactive Oxygen Species Formed by Secondary Organic Aerosols in Water and Surrogate Lung Fluid: H. Tong, et al.; Environ. Sci. Technol. 52, 11651 (2018), Abstract
Reactive oxygen species formed in aqueous mixtures of secondary organicaerosols and mineral dust influencing cloud chemistry and public health in the Anthropocene: H. Tong, et al.; Faraday Discuss. 200, 251 (2017), Application(s): ROS detection in aqueous mixtures of SOA by applying electron paramagnetic resonance (EPR) spectrometry, Abstract — Full Text
Oxidation of the Flavonolignan Silybin. In situ EPR Evidence of the Spin-Trapped Silybin Radical: R. Sokolova, et al.; Electrochim. Acta 205, 118 (2016), Application(s): in situ EPR spectroelectrochemistry
Mechanism of the hydroxyl radical oxidation of methacryloyl peroxynitrate (MPAN) and its pathway toward secondary organic aerosol formation in the atmosphere: T.B. Nguyen, et al.; Phys. Chem. Chem. Phys. 17, 17914 (2015), Application(s): Trapping agent of OH radicals, Abstract — Full Text
S-allyl cysteine alleviates nonsteroidal anti-inflammatory drug-induced gastric mucosal damages by increasing cyclooxygenase-2 inhibition, heme oxygenase-1 induction, and histone deacetylation inhibition: J.M. Park, et al.; J. Gastroenterol. Hepatol. 29 Suppl. 4, 80 (2014), Application(s): Electron spin resonance (ESR) spectroscopy, Abstract