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Bim_S/EL/L monoclonal antibody (3C5)

ALX-804-527

19 citations

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This antibody is covered by our Worry-Free Guarantee.

BimS/EL/L monoclonal antibody (3C5) Western blot — **Figure A:** Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the rat anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.Figure B: Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) recognizes all known isoforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), which detected EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 MAb was used as a loading control for all blots.Figure C: Anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 10⁶/lane) by Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) and detection by ECL. Probing with an anti-Hsp-70 MAb was used as a loading control.

Product Details

Alternative Name	BCL2L11
Application	ELISA, Flow Cytometry, ICC, IP, WB
Clone	3C5
Formulation	Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.
Host	Rat
Immunogen	Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.
Isotype	IgG2a
Purity Detail	Protein G-affinity purified.
Recommendation Dilutions/Conditions	Immunoprecipitation (2µg/ml)Western Blot (1-5µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Dog, Human, Monkey, Mouse, Rat
Specificity	Recognizes an epitope within aa 20-40 of im_L(~19kDa), Bim_EL(~23kDa), and Bim_S(~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region recently identified.
UniProt ID	O54918
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Circulating BiP/Grp78 is a novel prognostic marker for sepsis-mediated immune cell death.: Doerflinger, M., Reljić, B., et al.; FEBS J. 288, 1809 (2021), Application(s): WB / Reactant(s): Mouse, Abstract
MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis: M. Sochalska, et al.; Oncogene 36, 2066 (2017), Application(s): Immunoblotting, Abstract
CD80 Expressed by CD8+ T Cells Contributes to PD-L1-Induced Apoptosis of Activated CD8+ T Cells: M.R. Rollins, et al.; J. Immunol. Res. 2017, 7659462 (2017), Abstract — Full Text
Akt signaling is critical for memory CD8+ T-cell development and tumor immune surveillance: Rogel, A., Willoughby, J. E., et al.; PNAS 114, E1178 (2017), Abstract
Chemical chaperone TUDCA prevents apoptosis and improves survival during polymicrobial sepsis in mice: M. Doerflinger, et al.; Sci. Rep. 6, 34702 (2016), Application(s): Mouse cells, Abstract — Full Text
The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland: F. Schuler, et al.; Cell Death Differ. 23, 41 (2016), Application(s): Immunofluorescence microscopy, Abstract — Full Text
Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression: Hayakawa, K., Formica, A. M., et al.; J. Exp. Med. 213, 3007 (2016), Abstract
BAX-BAK1-independent LC3B lipidation by BH3 mimetics is unrelated to BH3 mimetic activity and has only minimal effects on autophagic flux: Reljić, B., Conos, S., et al.; Autophagy 12, 1083 (2016), Abstract
Intracellular localization of the BCL-2 family member BOK and functional implications: Echeverry, N., Bachmann, D., et al.; Cell Death Differ. 20, 785 (2013), Abstract
Elevated Mcl-1 inhibits thymocyte apoptosis and alters thymic selection: Campbell, K. J., Gray, D. H., et al.; Cell Death Differ. 19, 1962 (2012), Abstract

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Bim_S/EL/L monoclonal antibody (3C5)

ALX-804-527

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