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Bim_S/EL/L monoclonal antibody (14A8)

ALX-804-351

9 citations

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but not from Bim-deficient mice.""""" — **Figures:** IP/Western blot analysis of Bim expression in mouse (A) and human (C) cell lines. Bim proteins were immunoprecipitated from lysates of 10⁷ cells with anti-Bim_S/EL/L MAb (clone 14A8) (Prod. No. ALX-804-351) and anti-Bim_L MAb (clone 5E5) (Prod. No. ALX-804-352). Western blot analysis was performed with clones 14A8 and 5E5 followed by the addition of biotinylated mouse anti-rat IgG2b antibody and HRP-conjugated mouse anti-rat IgG2a antibody as the respective secondary antibodies

Product Details

Alternative Name	BCL2L11
Application	Flow Cytometry, ICC, IHC (PS), IP, WB
Clone	14A8
Formulation	Liquid. In PBS containing 10mM sodium azide.
Host	Rat
Immunogen	Recombinant mouse BimS.
Isotype	IgG2a
Purity Detail	Protein G-affinity purified.
Recommendation Dilutions/Conditions	Flow Cytometry (0.5-1µg/ml; overexpressing cell lines)Immunocytochemistry (1-5µg/ml)Immunohistochemistry (paraffin sections, 1-5µg/ml)Immunoprecipitation (1-2µg/ml)Western Blot (1-2µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Human, Monkey, Mouse
Specificity	Recognizes Bim_L, Bim_EL and Bim_S.
Technical Info / Product Notes	As Bim expression is generally low in non-transfected cell lines, please take care when choosing positive controls. Examples of cell lines which express detectable levels of Bim include: B6.2.16.BW2 or KO52.DA20 (T cell lymphomas) or ABL5.8.1 (B cell lymphoma) or MCF-7s. It is generally necessary to use a high amount of lysate (e.g. 1 x 10^7 cells). When using tissue samples, it is generally necessary to use 1-5mg of total protein from an appropriate tissue source.
UniProt ID	O54918
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The role of BH3-only protein Bim extends beyond inhibiting Bcl-2–like prosurvival proteins.: D. Mérino, et al.; J. Cell. Biol. 186, 355 (2009), Application(s): WB post IP, Abstract
Memory T-lymphocyte survival does not require T-cell receptor expression: Leignadier, J., Hardy, M. P., et al.; PNAS 105, 20440 (2008), Abstract
Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells: Y. Zhu, et al.; PNAS 101, 7681 (2004), Abstract — Full Text
BH3-only Bcl-2 family member Bim is required for apoptosis of autoreactive thymocytes: P. Bouillet, et al.; Nature 415, 922 (2002), Abstract
Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis: G.V. Putcha, et al.; Neuron 29, 615 (2001), Abstract
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O’Reilly, et al.; Am. J. Pathol. 157, 449 (2000), Abstract — Full Text
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science 286, 1735 (1999), Abstract
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell 3, 287 (1999), Abstract
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O’Reilly, et al.; Biotechniques 25, 824 (1998), Abstract

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Bim_S/EL/L monoclonal antibody (14A8)

ALX-804-351

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