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Western blot analysis of BAFF (human), mAb (Buffy-1) (Prod. No. ALX-804-128): Lane 1: MW marker; Lane 2: BAFF (soluble) (human), (recombinant) (Prod. No. ALX-522-025).
Schematic structure of human soluble BAFF (rec.) (aa 83-285). Recognizes the stalk region present in the membrane-bound form of hBAFF.
Membrane-bound human BAFF detected by MAb to BAFF (human) (Buffy-1) (Prod. No. ALX-804-128). Left panel: ICC; Right panel: phase contrast. Lower panel: 293-wt control.”””,BAFF (human) monoclonal antibody (Buffy-1) image,BAFF (human) monoclonal antibody (Buffy-1) image,yes
ALX-804-128,804-128-4.gif,http://static.enzolifesciences.com/fileadmin/files/image/804-128-4.gif,Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected
Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4µg/ml of MAb to BAFF (human) (Buffy-1). After washing in FACS buffer, FITC-conjugated antibody to rat IgG(ALX-211-051) was added and cells were analyzed by flow cytometry.
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
| Alternative Name | THANK, zTNF4, TALL-1, BlyS, TNFSF 13B/20, CD257 |
|---|---|
| Purity | ≥95% (SDS-PAGE) |
| Source | Produced in E. coli. The extracellular domain of human BAFF (aa 83-285) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag. |
| Alternative Name | B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
|---|---|
| Application | Flow Cytometry, ICC, WB |
| Host | Mouse |
| Isotype | IgG2a |
| Species Reactivity | Human |