AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
- Hot start technology prevents primer-dimers formation, improving sensitivity and specificity
- Better performance for amplification of GC-rich targets compared to competitors’ product
- Separate vial of ROX allows for flexibility of usage. The qPCR mix can be used without the reference dye ROX, with low concentration of ROX, and for high ROX amplification
- Consistent and reliable lot-to-lot performance, delivering reproduce results
AMPIGENE® qPCR Green Mix with Separate ROX uses the latest developments in polymerase technology and buffer chemistry to enhance qPCR speed, yield, and specificity. AMPIGENE® qPCR Green Mix with Separate ROX uses advanced hot-start technology for superior sensitivity.
AMPIGENE® qPCR Green Mix with Separate ROX contains AMPIGENE® Hot Start Taq DNA Polymerase enzyme, PCR green dye, dNTPs, MgCl, DMSO and propriety enhancers, enabling high sensitivity and high-fidelity qPCR of a wide range of targets and fragment sizes. The buffer system allows the efficient amplification of GC-rich and AT-rich templates under standard cycling conditions.
The AMPIGENE® ROX passive reference dye is supplied in a separate vial at 50X concentration.
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
Figure 3. Amplification results for the GC-rich DNA target were obtained by targeting a human genomic region that contains 72.9% GC content. Reactions were run in triplicate on a QuantStudio™ 5 real-time PCR system. The reactions with competitors’ reagents were included and the performances were assessed. Product from competitor B failed the amplification.
Figure 1A. Amplification results for the breast cancer gene 2 (BRCA2) were obtained from human genomic DNA samples in absence of ROX reference dye. Reactions were run in triplicate on a QuantStudio™ 5 real-time PCR system and each test was repeated three times. The reactions with competitor’s product were included in each test and the performance was assessed. AMPIGENE® qPCR Green Mix with Separate ROX shows higher sensitivity in comparison with the competitor’s product.
Figure 1B. Amplification results for the breast cancer gene 2 (BRCA2) were obtained from human genomic DNA samples with low ROX reference dye. Reactions were run in triplicate on a QuantStudio™ 5 real-time PCR system and each test was repeated three times. The reactions with competitor’s product were included in each test and the performance was assessed. AMPIGENE® qPCR Green Mix with Separate ROX shows higher sensitivity in comparison with the competitor’s product.
Figure 1C. Amplification results for the breast cancer gene 2 (BRCA2) were obtained from human genomic DNA samples with high ROX reference dye. Reactions were run in triplicate on a QuantStudio™ 5 real-time PCR system and each test was repeated three times. The reactions with competitor’s product were included in each test and the performance was assessed. AMPIGENE® qPCR Green Mix with Separate ROX shows higher sensitivity in comparison with the competitor’s product.
Figure 2C. Amplification results for the Transforming growth factor beta receptor 1 (TGFBR1) gene were obtained from human genomic DNA samples with high ROX reference dye. Reactions were run in triplicate on a QuantStudio™ 5 real-time PCR system and each test was repeated three times. The reactions with competitor’s product were included in each test and the performance was assessed. AMPIGENE® qPCR Green Mix with Separate ROX shows higher sensitivity in comparison with the competitor’s product.
Figure 2B. Amplification results for the Transforming growth factor beta receptor 1 (TGFBR1) gene were obtained from human genomic DNA samples with low ROX reference dye. Reactions were run in triplicate on a QuantStudio™ 5 real-time PCR system and each test was repeated three times. The reactions with competitor’s product were included in each test and the performance was assessed. AMPIGENE® qPCR Green Mix with Separate ROX shows higher sensitivity in comparison with the competitor’s product.
Figure 2A. Amplification results for the Transforming growth factor beta receptor 1 (TGFBR1) gene were obtained from human genomic DNA samples in absence of ROX reference dye. Reactions were run in triplicate on a QuantStudio™ 5 real-time PCR system and each test was repeated three times. The reactions with competitor’s product were included in each test and the performance was assessed. AMPIGENE® qPCR Green Mix with Separate ROX shows higher sensitivity in comparison with the competitor’s product.
| Regulatory Status |
RUO – Research Use Only |
|---|
Resources
Metabolism
Download Resource
Cellular Therapy
Download Resource
Cardiovascular Research
Download Resource
Drug Discovery
Download Resource
AMPIVIEW® RNA Probes
Download Resource
Immuno-Oncology
Download Resource
AMPIGENE® PCR Solutions
Download Resource
Bioprocess
Download ResourceRelated Products
AMPIGENE® 1-Step RT-PCR Kit
ENZ-KIT105
Optimized and efficient cDNA synthesis and PCR in a single tube
| Application | PCR |
|---|
| Application | PCR |
|---|
AMPIGENE® HS Taq Mix
ENZ-NUC101
Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity
| Application | PCR |
|---|