Home Shop 5′-Nucleotidase (from Crotalus atrox venom)

5′-Nucleotidase (from Crotalus atrox venom)

BML-KI307

Datasheet
Manual
SDS
CofA

Already have an account?

5’-nucleotidase (5’-ribonucleotide phosphohydrolase; EC 3.1.3.5) is partially purified from Crotalus atrox (Western Diamondback Rattlesnake) venom and is free of detectable phosphodiesterase activity.

Shipping: Available products typically ship within 24/48h, via priority shipping.

Do you need support? Contact Customer Service or Technical Support.

Online Account
Access or Create Your Account

Product Details

Activity	One U will release 1pmol phosphate per minute from 200µM 5’-AMP, 30°C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2.
Application Notes	5’-nucleotidase is useful as the second enzyme in coupled assays of phosphodiesterases (3’,5’-cyclic nucleotide phosphodiesterase; EC 3.1.4.17). Phosphodiesterase catalyzed hydrolysis of, for example, cAMP to 5’-AMP is coupled to 5’-nucleotidase cleavage of 5’-AMP to inorganic phosphate and adenosine. Phosphodiesterase activity is then quantified by colorimetric detection of the inorganic phosphate with a reagent such as BIOMOL Green (Prod. No. BML-AK111; see also Cyclic Nucleotide Phosphodiesterase Assay Kit, Prod. No. BML-AK800).
Formulation	Liquid. In TRIS-HCl, pH 7.4, 150mM sodium chloride, 1mM magnesium chloride and 10% (v/v) glycerol.
Quantity	5000kU in 1000µl

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Stable for at least 6 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing). Frozen storage of smaller aliquots is recommended if more than 6 freeze-thaws of the entire vial contents (1000µl) are anticipated. Thawed enzyme is stable on ice for at least 2 hrs.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Heme-Edge Residues Modulate Signal Transduction within a Bifunctional Homo-Dimeric Sensor Protein: D.C. Patterson, et al.; Biochemistry 60, 3801 (2021), Abstract
Single inhibition of either PDE3 or PDE4 unmasks β2-adrenoceptor-mediated inotropic and lusitropic effects in the left but not right ventricular myocardium of rat: F. Soler, et al.; Eur. J. Pharmacol. 765, 429 (2015), Abstract

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Specific Protocol

5000 kU is sufficient for 100 coupled phosphodiesterase assays (50'000 U/10µl per well). Coupled reactions carried out in a final reaction volume of 50µl with 0.25mU phosphodiesterase (1 mU = 1nmol 3’,5’-cAMP to 5’-AMP per minute at pH 7.4, 30°C) in 10mM Tris-HCl, pH 7.4, 0.2mM MgCl₂, 200µM 3’,5’-cAMP, 30°C. See protocol for Cyclic Nucleotide Phosphodiesterase Assay Kit, Prod. No. BML-AK800, for assay details.

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

Enzo Life Science provides GHS Compliant SDS

If your language is not available please fill out the SDS request form

Your Cart