5-N₃Ac-AA-dUTP

The azide-containing 5-N₃Ac-AA-dUTP is an analogue of dUTP and a suitable molecular tool for the copper(I)-catalysed Huisgen azide-alkyne [3+2] cycloaddition (CuAAC) or copper(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) with strained alkynes, respectively. Both reactions have attracted particular interest for the introduction of reporter groups and for the labelling of various biomolecules like TTP-binding proteins by the so-called click-chemistry technique. 5-Azide-functionalised pyrimidine nucleotides can replace their natural counterpart TTP in polymerase reactions, leading to the generation of a modified and functionalized DNA strand that can subsequently be further reacted and modified using click chemistry methods.