Home Shop 1α,25-Dihydroxyvitamin D3

1α,25-Dihydroxyvitamin D3

BML-DM200

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Active form of vitamin D

Ultra-high purity
Used in bone, immunology, and metabolism studies
Highly cited

1α,25-Dihydroxyvitamin D₃ is the active hormonal form of vitamin D. It inhibits proliferation and induces differentiation of a variety of cells including osteoclasts and HL-60 cells. It is a central regulator of bone and calcium homeostasis. It inhibits proliferation and induces differentiation of a variety of cells including osteoclasts, keratinocytes, HL-60 cells, vascular smooth muscle cells, and bone marrow macrophage precursors. It can activate signal transduction pathways that include hydrolysis of phosphatidylcholine and formation of diacylglycerol in chick myoblasts, and activation of Raf kinase in rat hepatic primary cells and 3T3 fibroblasts. It regulates c-myc proto-oncogene transcription, IL-8 expression, cyclooxygenase induction, and directly activates protein kinase C at physiological concentrations (EC₅₀=16 nM). It up-regulates RANKL level and down-regulates OPG level.

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Product Details

Alternative Name	1α,25-Dihydroxycholecalciferol, Calcitriol
Appearance	White solid.
CAS	32222-06-3
Couple Target	RANKL, Vitamin D receptor
Couple Type	Activator, Ligand
Formula	C₂₇H₄₄O₃
Identity	Identity determined by MS and NMR.
MW	416.6
Purity	≥95% (HPLC)
Solubility	Soluble in acetone, chloroform, DMSO, 100% ethanol, ethyl acetate, or tetrahydrofuran. Evaporative loss of solvent may occur during storage and handling of the dissolved product. If necessary, exact concentration may be re-determined spectrophotometrically.
Source	Synthetic.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light. For maximum stability, store solid in the dark under an inert gas such as argon. Store dissolved product in the dark in a tightly sealed container.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Serum vitamin D deficiency is associated with increased risk of γδ T cell exhaustion in HBV-infected patients: Li, K., Lu, E., et al.; Immunology 171, 31 (2024), Abstract
Effects of 1,25-Vitamin D3 and 24,25-Vitamin D3 on Corneal Nerve Regeneration in Diabetic Mice: X. Lu, et al.; Biomolecules 13, 1754 (2023), Abstract
Vitamin D3 Exerts Beneficial Effects on Aging Muscles through Activation of the Vitamin D Receptor (VDR)/Sirtuin (SIRT)1/3 Axis: N.F. Talib, et al.; Nutrients 15, 4717 (2023), Abstract
Effects of Topical 1,25 and 24,25 Vitamin D on Diabetic, Vitamin D Deficient and Vitamin D Receptor Knockout Mouse Corneal Wound Healing: X. Lu, et al.; Biomolecules 13, 1065 (2023), Abstract
Development of a rapid in vitro pre-screen for distinguishing effective liposome-adjuvant delivery systems: L.A.J. Feather, et al.; Sci. Rep. 12, 12448 (2022), Abstract
1,25-Dihydroxyvitamin D3 suppresses CD4+ T-cell effector functionality by inhibition of glycolysis: E.L. Bishop et al.; Immunology 166, 299 (2022), Abstract
1α,25(OH)2D3 reverses exhaustion and enhances antitumor immunity of human cytotoxic T cells: P. Li, et al.; J. Immunother. Cancer 10, e003477 (2022), Abstract — Full Text
Asprosin exerts pro-inflammatory effects in THP-1 macrophages mediated via the Toll-like receptor 4 (TLR4) pathway: K. Shabir, et al.; Int. J. Mol. Sci. 24, 227 (2022), Abstract — Full Text
Molecular mechanisms of bifunctional vitamin D receptor agonist-histone deacetylase inhibitor hybrid molecules in triple-negative breast cancer: C. Barbier, et al.; Sci. Rep. 12, 6745 (2022), Abstract — Full Text
CRISPR Interference Reveals That All-Trans-Retinoic Acid Promotes Macrophage Control of Mycobacterium tuberculosis by Limiting Bacterial Access to Cholesterol and Propionyl Coenzyme A: G. H. Babunovic, et al.; Mbio 13, e0368321 (2022), Abstract