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What are the excitation and emission spectra of the FLUOR DE LYS® substrate?
The fluorophore is excited with 360nm light and the emitted light is detected at 460nm on a fluorometric microplate reader.…
Tue, Jul 18, 2023
Can you measure the activity of SIRT1 with the FLUOR DE LYS® HDAC fluorometric activity assay kit?
This kit measures the activity of all HDACs provided with a biological sample. Simply changing the substrate from the FLUOR…
Tue, Jul 18, 2023
What is the recommended substrate for the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit?
The FLUOR DE LYS® SIRT1 substrate, the recommended substrate, is provided in the kit. The FLUOR DE LYS® SIRT1 substrate is a…
Tue, Jul 18, 2023
Does the standard included in BML-AK555 need to be incubated with recombinant SIRT1?
The deacetylated standard included in the kit can be used to create a standard curve giving the relationship between deacetylated…
Tue, Jul 18, 2023
How should KillerTRAIL™ and SuperKillerTRAIL™ be handled and stored to best assure stability of their biological activity?
Once thawed, it is recommended that appropriate aliquots are prepared and stored at -80°C. In order to retain full activity,…
Tue, Jul 18, 2023
Will EDTA or EGTA inhibit SIRT1 activity?
We have not specifically tested metal chelators on the sirtuins. Reaction inhibition by EDTA or EGTA is possible although inhibition…
Tue, Jul 18, 2023
Why is SuperKillerTRAIL™ much more effective in inducing apoptosis than KillerTRAIL™ on certain cell types?
Apoptosis induction by TRAIL is triggered via binding to TRAIL-Receptors 1 and 2 on the cell surface. Depending on cell…
Tue, Jul 18, 2023
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023
What is the amount or concentration of SIRT1 provided in BML-AK555?
The volume or concentration of SIRT1 is lot-dependent. Each kit contains 100 units of the enzyme. Concentration on specific lot of SIRT1 is available…
Tue, Jul 18, 2023
Can you measure HDAC activity in cellular samples?
Yes, our FLUOR DE LYS® fluorometric activity assay kit (BML-AK500) has been developed to work with tissue/cell lysates, immunoprecipitates or…
Tue, Jul 18, 2023