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I am working with rabbit sera. Can I use your Oxytocin ELISA kit for detecting oxytocin in this sample?
Problems may arise when working with rabbit sera because the kit relies on the use of an anti-rabbit IgG. This…
Tue, Jul 18, 2023
Is this Oxytocin ELISA kit species-independent?
Although novel forms of oxytocin with a single amino acid substitution, which may affect overall antibody binding affinity, have been…
Tue, Jul 18, 2023
What are the differences between TRAIL, KillerTRAIL™ and SuperKillerTRAIL™?
To induce apoptosis, TRAIL needs to form multimers, showing increasing activity with increasing multimerization levels. Multimerization of TRAIL can be…
Tue, Jul 18, 2023
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
What is the recommended assay concentration range for KillerTRAIL™ and SuperKillerTRAIL™?
The recommended working dilution range for KillerTRAIL™ is 10-100 ng/ml, and 2-100 ng/ml for SuperKillerTRAIL™. However, adjustment may be necessary for the end-user’s specific…
Tue, Jul 18, 2023
Will EDTA or EGTA inhibit SIRT1 activity?
We have not specifically tested metal chelators on the sirtuins. Reaction inhibition by EDTA or EGTA is possible although inhibition…
Tue, Jul 18, 2023
How should KillerTRAIL™ and SuperKillerTRAIL™ be handled and stored to best assure stability of their biological activity?
Once thawed, it is recommended that appropriate aliquots are prepared and stored at -80°C. In order to retain full activity,…
Tue, Jul 18, 2023
What is the amount or concentration of SIRT1 provided in BML-AK555?
The volume or concentration of SIRT1 is lot-dependent. Each kit contains 100 units of the enzyme. Concentration on specific lot of SIRT1 is available…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023